Maize
Summary
The University of
Toledo (US), Pioneer Hi-Bred (US), and Stine
Biotechnology (US) have been granted United States patents directed to
Agrobacterium-mediated transformation of maize. The main difference
among them is the type of maize tissue used as target for transformation:
- The University of Toledo claims a maize seedling wounded in a specific area for inoculation of the bacterium.
- Pioneer Hi Bred claims an immature embryo of maize and a medium formula for cultivation of a transformed maize embryo
- Stine Biotechnology also employs immature embryos as target tissue for transformation with Agrobacterium in their patent. Claims in their applications disclose a method to improve the production of so-called Type II callus (a preferred target tissue for the transformation of corn) by including a monosaccharide like glucose in addition to the usual sucrose in the medium.
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