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Patent Lens > Technology Landscapes > Agrobacterium-mediated transformation of plants

Rice

Summary

  • long_rice_grainUnited States and Australian patents were granted to Japan Tobacco on methods for the transformation of Indica rice. Patents are pending in other jurisdictions. The patent specification discloses the use of immature embryo cells of Indica rice for transformation with Agrobacterium spp. The claims granted recite wide ranges for typical components of media used in the selection medium. Note that other varieties of rice, such as Japonica rice, are not covered by the claims as granted (Update July 2003).
  • In 2001, the National Science Council of R.O.C. in Taiwan was granted a United States patent directed to a method for the transformation of immature rice embryos with Agrobacterium. The invention is not limited to any particular rice variety. A particular feature of the transformation method is that the rice embryos and Agrobacterium are co-cultivated with a dicot suspension culture. In one of the preferred embodiments, the dicot suspension culture is made of potato cells. According to the inventors, such culture is rich in phenolic compounds, which induce the vir genes of the Ti plasmid. Thus, the phenolic compounds of the culture assist the transformation process.
  • Paradigm Genetics filed a PCT application disclosing the transformation of a rice panicle with Agrobacterium using vacuum infiltration. This method is carried out in planta (the transformed plant part is still attached to the whole plant) and avoids in vitro regeneration steps.
  • The Indian company Avestha Gengraine Technologies has filed a PCT application disclosing a method of transformation of Indica rice using excised shoot tip tissue as a target for Agrobacterium.

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