EP Patent application filed by the United States Department of Agriculture (USDA)

A method for producing a transgenic plant by:

A) applying Agrobacteria having a foreign gene to a pollen culture on a solid medium;
B) allowing germination and growth of the transgenic pollen on the medium;
C) fertilizing a second plant with the transgenic pollen;
D) obtaining seed; and
E) germinating the seed to obtain a transgenic plant.

A medium for pollen germination and pollen tube growth having agarose, sucrose, KNO₃, MnSO₄, H₃BO₃, MgSO₄, and gibberellic acid.

A method for producing a transgenic plant comprising:

A) obtaining pollen from a first plant;
B) applying a lawn of Agrobacteria to a solid pollen culture medium, the Agrobacteria comprising at least one heterologous gene sequence capable of being transferred to a plant cell;
C) applying the pollen to the solid medium;
D) allowing the pollen to germinate and grow on the medium, thereby producing transgenic pollen;
E) applying the transgenic pollen to the stigma of a second plant capable of being fertilized by the pollen of the first plant, thereby fertilizing the second plant;
F) obtaining transgenic seed from the second plant;
G) germinating the transgenic seed to obtain a transgenic plant.

Claim 8**

A medium for pollen germination and pollen tube growth comprising agarose, sucrose, KNO₃, MnSO₄ , H₃BO₃, MgSO_4, and gibberellic acid.