Banana (Musa) - Specific Patent
Information - part 1
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5792935
- Earliest priority - 9 December 1993
- Filed - 9 December 1994
- Granted - 11 August 1998
- Expected expiry - 10 August 2015
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Title - Agrobacterium tumefaciens transformation of
Musa species
| Claim 1
A method for transforming a Musa plant, said method comprising:
A) wounding meristematic tissue from a Musa plant to generate a
wounded Musa plant tissue and to facilitate access of
Agrobacterium tumefaciens to Musa plant cells competent for
transformation and regeneration; and
B) applying to said wounded
Musa plant tissue at least one transformation competent
Agrobacterium tumefaciens to transform said Musa plant,
wherein said at least one transformation competent Agrobacterium
tumefaciens harbors at least one Ti plasmid and at least one virulence
gene, wherein said at least one Ti plasmid comprises at least one genetically
engineered T-DNA to effect transformation of said Musa plant.
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Transformation of an apical or adventitious meristem of a Musa
plant by wounding the tissue and inserting A. tumefaciens carrying a
T-DNA with foreign DNA. The plant can be transformed for the production of
pharmaceutical products or the alteration of phenotypic traits of the fruit.
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Texas A & M University
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AU
693506 B2
- Earliest priority - 9 December 1993
- Filed - 9 December 1994
- Granted - 2 July 1998
- Expected expiry - 8 December 2014
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Title - Agrobacterium tumefaciens transformation of
Musa species
| Claim 1
A method for transforming a Musa plant, said method including:
A) wounding meristematic tissue from a Musa plant, followed by
bombarding the said wounded tissue with microparticles, to generate a wounded
Musa plant tissue and to facilitate access of Agrobacterium
tumefaciens to Musa plant cells competent for transformation and
regeneration; and
B) applying to said wounded Musa plant tissue at
least one transformation competent Agrobacterium tumefaciens to
transform said Musa plant, wherein said at least one transformation
competent Agr obacterium tumefaciens harbors at least one Ti plasmid
and at least one virulence gene, wherein said at least one Ti plasmid includes
at least one genetically engineered T-DNA to effect transformation of said
Musa plant.
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Microbombardment of wounded meristematic tissue of a Musa plant to
facilitate A. tumefaciens infection. The transformation method is used
to obtain Musa plants producing pharmaceutical products and fruits with
improved phenotypic traits.
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EP 731632 B1
- Earliest priority - 9 December 1993
- Filed - 9 December 1994
- Granted - 7 November 2001
- Expected expiry - 8 December 2014
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Title - Agrobacterium tumefaciens transformation of
Musa species
| Claim 1
A method for transforming a Musa plant, said method comprising:
A) pre-wounding the meristematic tissue from a Musa plant prior to
bombarding said plant with microparticles;
B) wounding the pre-wounded
meristematic tissue by microparticle bombardment to generate a wounded
Musa plant tissue and to facilitate access of Agrobacterium
tumefaciens to Musa plant cells competent for transformation and
regeneration; and
B) applying to said wounded Musa plant tissue at
least one transformation competent Agrobacterium tumefaciens to
transform said Musa plant, wherein said at least one transformation
competent Agrobacterium tumefaciens harbors at least one Ti plasmid and
at least one virulence gene, wherein said at least one Ti plasmid comprises at
least one genetically engineered T-DNA to effect transformation of said
Musa plant.
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Designated contracting States at the time of grant are: Austria, Belgium,
Germany, Spain, France, United Kingdom, Greece (reported on INPADOC as lapsed),
Ireland, Italy, Netherlands, Portugal, Sweden.
A method for transforming a Musa meristematic tissue similar to the
method disclosed in the related Australian patent. The tissue is wounded prior
to the wounding by microbombardment. Double wounding of the tissue facilitates
access of A. tumefaciens to Musa plant cells.
The claims
as filed of the EP application had disclosed a single wounding step by
microbombardment. The granted claims are more limiting as a prior wounding step
is part of the method.
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CA 2177267 A
- Earliest priority - 9 December 1993
- Filed - 9 December 1994
- Granted - 28 September 2004
- Expected expiry - 8 December 2014
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Title - Agrobacterium tumefaciens transformation of
Musa species
| Claim 1
A method for transforming a Musa plant, said method comprising:
A) wounding meristematic tissue from a Musa plant by microparticle
bombardment to generate a wounded Musa plant tissue and to facilitate
access of Agrobacterium tumefaciens to Musa plant cells
competent for transformation and regeneration; and
B) applying to said
wounded Musa plant tissue at least one transformation competent
Agrobacterium tumefaciens to transform said Musa plant,
wherein said at least one transformation competent Agrobacterium
tumefaciens harbors at least one Ti plasmid and at least one virulence
gene, wherein said at least one Ti plasmid comprises at least one genetically
engineered T-DNA to effect transformation of said Musa plant.
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Claims as filed are similar to the Australian patent but a single step of
wounding of the Musa tissue by microbombardment prior to the
transformation with A. tumefaciens is disclosed.
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EP
1087016 A2
- Earliest priority - 9 December 1993
- Filed - 9 December 1994
- Deemed to be withdrawn - 10 August 2005
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Title - Agrobacterium tumefaciens transformation of
Musa species
Claim 1
A method for transforming a Musa plant, said method comprising:
A) wounding meristematic tissue from a Musa plant by microparticle
bombardment to generate a wounded Musa plant tissue and to facilitate
access of Agrobacterium tumefaciens to Musa plant cells
competent for transformation and regeneration; and
B) applying to said
wounded Musa plant tissue at least one transformation competent
Agrobacterium tumefaciens to transform said Musa plant,
wherein said at least one transformation competent Agrobacterium
tumefaciens harbors at least one Ti plasmid and at least one virulence
gene, wherein said at least one Ti plasmid comprises at least one genetically
engineered T-DNA to effect transformation of said Musa plant;
C)
growing said transformed Musa plant for a sufficient time to identify
the presence of chimeric features;
D) producing non-chimeric tissue by
dividing said transformed Musa plant into segments which have at least
one meristem which can regenerate into an intact plant and which have cells that
are uniformily transformed to produce non-chimeric tissue; and
E) growing
said non-chimeric tissue into a non-chimeric plant.
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This application was a divisional application to the application EP 731632
A1. Microbombardment is also used to wound a meristematic tissue of a Musa plant
prior to the transformation with A. tumefaciens. In addition, the
transformed Musa tissue is grown to identify chimeras and regenerate an intact
plant from non-chimeric tissue.
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Remarks
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Application filed in Japan (JP 9508786 T2) is deemed to be withdrawn.
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Note: Patent information on this page was last updated on 17 February 2006.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
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