Patent application filed by the University of Guelph (Canada)
The present disclosure is directed to transformation of any explant from
monocot plants using Agrobacterium. According to the applicants, an
advantage of the disclosed method is that vacuum infiltration of the tissue in
the presence of Agrobacterium and a phenolic compound such as
acetosyringone yields better transformation efficiency than by wounding.
According to the inventors, the method is also simpler than previously described
methods. Because the known patent applications have lapsed, the disclosed
methods may be in the public domain. If you'd like to use it and are uncertain
that it is in the public domain in your jurisdiction, it may be best to
contact the
assignee, for which a recent contact address disclosed on the website is
plorenz@uoguelph.ca.
Specific PCT Application Information
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PCT Number
|
Title, Independent Claims and Summary of Claims
|
Assignee
|
|
WO
00/58484 A2
- Earliest priority - 26 March 1999
- Filed - 26 September 2001
- OPI - 5 October 2000
- Expected expiry - N/A
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Title - Transformation of monocotyledoneous plants using
Agrobacterium
| Claim 1
A method for the transformation of a monocot plant comprising,
i)
exposing explant tissue of said monocot plant to an Agrobacterium
strain under vacuum in the presence of a phenolic compound, said
Agrobacterium strain comprising a heterologous gene of interest within
a vector;
ii) removing said Agrobacterium from said explant
tissue;
iii) adding an antibiotic against said Agrobacterium;
and
iv) selecting explant tissue for occurrence of said heterologous gene
of interest.
|
| Claim 10
A method for the transformation of a monocot plant comprising,
i)
placing explant tissue of said monocot plant into media comprising a suspension
of Agrobacterium to obtain a mixture, said Agrobacterium
strain comprising a heterologous gene of interest within a vector;
ii)
maintaining said mixture under vacuum in the presence of acetosyringone;
iii) releasing said vacuum and further incubating said explant tissue in the
presence of said Agrobacterium;
iv) transferring said explant
tissue to fresh media comprising acetosyringone and incubating said explant
tissue in the dark
v) washing said explant tissue with an antibiotic
against said Agrobacterium,
vi) transferring said explant tissue
to fresh media and allowing said explant tissue to differentiate, thereby
producing differentiated calli;
vii) placing said differentiated calli onto
media containing a selection agent, and maintaining said differentiated calli in
the light; and
viii) obtaining calli that grow in the presence of the
selection agent.
|
| Claim 17
A method for the transformation of a monocot plant comprising,
i)
placing explant tissue of said monocot plant into media comprising a phenolic
compound, and a suspension of Agrobacterium to obtain a mixture, said
Agrobacterium strain comprising a heterologous gene of interest within
a vector;
ii) washing said explant tissue with an antibiotic against said
Agrobacterium and transferring said explant tissue to fresh media
comprising acetosyringone and incubating said explant tissue in the dark;
iii) transferring said explant tissue to fresh media and allowing said explant
tissue to differentiate, thereby producing differentiated calli;
iv)
placing said differentiated calli to media containing a selection agent, and
maintaining said differentiated calli in the light; and
v) obtaining calli
that grow in the presence of the selection agent.
|
The present PCT application recites:
- method of transforming monocot plant
- by vacuum infiltration of a plant tissue
- with Agrobacterium having a heterologous gene of interest on a
vector
- in the presence of a phenoloic compound, e.g. acetosyringone.
|
University of Guelph
(Canada)
|
| Remarks |
Related applications in Australia (AU 112261/00), Canada (CA 2368841), and US
(US
2002/112261) have lapsed or been withdrawn.
|
Note: Patent information on this page was last updated on 5 February 2006.
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