Immature embryo transformation - patents and application assigned to Japan Tobacco
Patents and application assigned to Japan Tobacco
This family of patents discloses use of an immature embryo of a monocot for Agrobacterium transformation. Within the embryo, the scutellum (name given to the single massive cotyledon (seed leaf) of monocot plants) is transformed. The scutellum is capable of producing dedifferentiated calli having the ability to regenerate normal plants after transformation.
The bacterium used for transformation contains either a Ti or Ri (root-inducing) plasmid with the desired gene and a plasmid having a virulence region derived from the A. tumefaciens Ti plasmid pTiBo542.
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