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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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AU733080
B2
- Earliest priority - 15 July 1997
- Filed - 14 July 1998
- Granted - 3 May 2001
- Expected expiry - 14 July 2018
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Title - Pollen-based transformation system using solid media
Claim 1
A method for producing a transgenic plant comprising:
a. obtaining pollen from a first plant,
b. applying a lawn of
Acrrobacteria to a solid pollen culture medium, the
Agrobacteria comprising at least one heterologous gene sequence capable
of being transferred to a plant cell,
c. applying the pollen to the solid
medium,
d. allowing the pollen to germinate and grow on the medium, thereby
producing transgenic pollen,
e. applying the transgenic pollen to the
stigma of a second plant capable of being fertilized by the pollen of the first
plant, thereby fertilizing the second plant,
f. obtaining transgenic seed
from the second plant,
g. germinating the transgenic seed to obtain a
transgenic plant.
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Claim 8
A medium for pollen germination and pollen tube growth comprising agarose,
sucrose, KNO3, MnSO4, H3BO3,
MgSO4, and gibberellic acid.
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The Australian patent 733 080 claims the same method for transforming pollen
of a plant with Agrobacterium as the United States patent. However, the
Australian patent claims in addition a specific medium for pollen germination
and pollen tube growth.
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United States Department of Agriculture (USDA)
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EP
996328 B1
- Earliest priority - 15 July 1997
- Filed - 14 July 1998
- Granted - 5 March 2003
- Expected expiry - 14 July 2018
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Title - Pollen-based transformation system
using solid media
Claim 1
A method for producing a transgenic plant comprising:
a. obtaining pollen from a first plant,
b. applying a lawn of
Agrobacteria to a solid pollen culture medium, the
Agrobacteria comprising at least one heterologous gene sequence capable
of being transferred to a plant cell,
c. applying the pollen to the solid
medium,
d. allowing the pollen to germinate and grow on the medium, thereby
producing transgenic pollen,
e. applying the transgenic pollen to the
stigma of a second plant capable of being fertilized by the pollen of the first
plant, thereby fertilizing the second plant,
f. obtaining transgenic seed
from the second plant,
g. germinating the transgenic seed to obtain a
transgenic plant.
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The European patent 996328 claims the same method for transforming pollen of
a plant with Agrobacterium as the United States patent.
Designated contracting States at the time of grant are: Austria, Belgium,
Switzerland, Cyprus, Germany, Denmark, Spain, Finland, France, United Kingdom,
Greece, Ireland, Italy, Liechtenstein, Luxembourg, Monaco, Netherlands,
Portugal, Sweden
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US
5929300
- Earliest priority - 15 July 1997
- Filed - 15 July 1997
- Granted - 27 July 1999
- Expected expiry - 15 July 2017
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Title - Pollen-based transformation system
using solid media
Claim 1
A method for producing a transgenic plant comprising:
a. obtaining pollen from a first plant,
b. applying a lawn of Agrobacteria to a solid pollen culture medium,
the Agrobacteria comprising at least one heterologous gene sequence
capable of being transferred to a plant cell,
c. applying the pollen to the solid medium,
d. allowing the pollen to germinate and grow on the medium, thereby producing
transgenic pollen,
e. applying the transgenic pollen to the stigma of a second plant capable of
being fertilized by the pollen of the first plant, thereby fertilizing the
second plant,
f. obtaining transgenic seed from the second plant,
g. germinating the transgenic seed to obtain a transgenic plant.
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Delta and Pine Land Co., acquired exclusive licensing rights to the
pollen-transformation system developed by the USDA in the United States.
(Source: Ag Biotech InfoNet, January 26, 2001).
The United States patent 5 929 300 claims:
- a method for producing a transgenic plant by transforming pollen of a plant
with Agrobacterium carrying a foreign gene and then fertilizing a second plant
with the transgenic pollen to obtain transgenic seed. The transgenic plant is
obtained by germination of the transgenic seeds. The Agrobacterium
must be applied as a lawn to a solid pollen culture medium and
the pollen must also be applied to the solid culture medium. Thus, it appears
that other methods of applying the Agrobacterium (e.g., by pipetting drops onto
the medium) might not fall within the scope of the claims.
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WO
1999/03326
- Earliest priority - 15 July 1997
- Filed - 14 July 1998
- OPI - 28 January 1999
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Title - Pollen-based transformation system
using solid media
Claim 1
A method for producing a transgenic plant comprising:
a. obtaining pollen from a first plant,
b. applying a lawn of
Acrrobacteria [sic] to a solid pollen culture medium, the Agrobacteria
comprising at least one heterologous gene sequence capable of being transferred
to a plant cell,
c. applying the pollen to the solid medium,
d.
allowing the pollen to germinate and grow on the medium, thereby producing
transgenic pollen,
e. applying the transgenic pollen to the stigma of a
second plant capable of being fertilized by the pollen of the first plant,
thereby fertilizing the second plant,
f. obtaining transgenic seed from the
second plant,
g. germinating the transgenic seed to obtain a transgenic
plant.
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Claim 8
A medium for pollen germination and pollen tube growth comprising agarose,
sucrose, KNO3, MnSO4, H3BO3, MgSO4, and gibberellic acid.
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| Remarks |
- National phase entry of WO 1999/03326 in Canada (CA 2296501) is pending.
- National phse entry of WO 1999/03326 in China (CN 1098029 C) has been
published as granted on 8 January 2003.
- Related patent documents also filed in Brazil (BR 9811791), The Hong Kong
Special Administrative Region of the People's Republic of China (HK 1026118),
Taiwan (TW 577922) and South Africa (ZA 9806240).
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