Seed Transformation - patent application assigned to Scigen Harvest

PCT patent application WO 02/066599 discloses a method to transform plants by wounding pre-germinated seeds with needles and treating the wounded areas with Agrobacterium tumefaciens containing a vector with the desired gene. The disclosure only describes wounding a cotyledonary node.

Specific Patent Application information

Patent Number

Title, Independent Claims and Summary of Claims

Assignee

WO 2002/066599

Earliest priority - 16 February 2001
Filed - 14 February 2002
OPI - 29 August 2002

Title - Efficient method for the development of transgenic plants by gene manipulation

Claim 1

A method for transforming plants, comprising the steps of:

1) selecting half a seed, which is germinated for one day as a target plant tissue;
2) wounding the target plant tissue using a bundle of needles;
3) inserting a target gene into the target plant tissue with Agrobacterium tumefaciens vector containing the target gene; and
4) regenerating a whole plant from the target plant tissue.

The claims cover

wounding half a seed which is selected and germinated for a day and are not limited to wounding the node.
In the examples, only soybean is transformed, but the independent claim is unrestricted in terms of plant family or species. Dependent claims recite a long list of commercially important crop plants, monocots and dicots.
Wounding must be done with a bundle of needles, though it's not clear how the needles are bundled (e.g., tied together? collected in some other fashion?) and how many needles you need to form a bundle (more than two?).
Step 3) in claim 1 recites '... with Agrobacterium tumefaciens vector...', the claim is not limited to the use of Agrobacterium to provide this vector, although the disclosure describes no other way of providing the vector.

The numerous limitations in independent Claim 1 suggest various ways of inventing around a patent that might issue with this claim language, e.g., by using other than half a seed, pre-germinating for more or less than a day, wounding with one needle or using two or more needles which are not bundled.

Scigen Harvest Co. Ltd

Remarks

Priority document published in Kuwait (KR 2002067303).

Note: Patent information on this page was last updated on 23 February 2006.

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