Seed Transformation - patent applications by The Agri-Biotechnology Research Center of Shanxi

The method disclosed in the following patent applications is based on wounding the apical meristem of the appearing bud in pre-germinated seeds. Although the patents only disclose how to transform one type of species, the independent claims cover methods of transforming plants from any family or species.

Specific Patent Information

Patent Number

Title, Independent Claims and Summary of Claims

Assignee

US 2002/0184663 A1

Earliest priority - 26 February 2001
Filed - 19 February 2002
Application pending

Title - Method of Agrobacterium mediated plant transformation through treatment of germinating seeds

Claim 1

A plant transformation method mediated by Agrobacterium comprising:

(a) providing germinating seeds; and

(b) co-culturing the germinating seeds with an Agrobacterium strain containing Ti plasmids with an inserted nucleic acid sequence so that the foreign nucleic acid sequence carried by the Agrobacterium strain is transferred and integrated into the genome of the germinating seeds through Ti plasmids.

United States application US 2002/0184663 discloses

a method to transform plants by treating pre-germinated seed with Agrobacterium tumefaciens containing Ti plasmids with a desired gene.

As described in the disclosure, the method is based on wounding the apical meristem of the appearing bud. Independent Claim 1 does not require wounding, merely contacting Agrobacterium containing a Ti plasmid having a desired gene with germinating seeds. The source of the seeds is unrestricted in terms of family or species of plants, whereas only maize seeds are exemplified in the patent text.

Dependent claims recite monocots and dicots.

Agri-Biotechnology Research Center of Shanxi

EP 1 236 801 A2

Earliest priority - 26 February 2001
Filed - 25 February 2002
Application deemed withdrawn - 2 January 2004

Title - Method of Agrobacterium mediated plant transformation through treatment of germinating seeds

Claim 1

A plant transformation method mediated by Agrobacterium, characterized in that germinating seeds were used as the receptors, an Agrobacterium strain containing Ti plasmids with an inserted nucleic acid sequence was used as the donor, the germinating seeds are co-cultured with the Agrobacterium strain so that the foreign nucleic acid sequence carried by the Agrobacterium strain can be transferred and integrated into the genome of the receptor through Ti plasmids.

Remarks

Related application in Canada (CA 2372793) is pending.
Related patent in China (CN 1149918) has been granted on 19 May 2004.

Note: Patent information on this page was last updated on 23 February 2006.

The information contained in this page was believed to be correct at the time it was collated. New patents and patent applications, altered status of patents, and case law may have resulted in changes in the landscape. CAMBIA makes no warranty that it is correct or up to date at this time and accepts no liability for any use that might be made of it. Corrections or updates to the information are welcome. Please send an email to info@bios.net.

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