Patents granted to Syngenta Mogen B.V.
The disclosed invention is directed to the transformation of dicots using an
Agrobacterium carrying binary vectors where the T-DNA is located in one
plasmid and the vir region is located in a different plasmid. The
separation of the T-DNA and the vir region on two different plasmids is
the basic principle of binary vector systems. The two United States patents are
still in force, while the European and Japanese patents have reached their
maximum 20 year protection term from filing and therefore have expired.
Specific Patent Information
|
Patent Number
|
Title, Independent Claims and Summary of Claims
|
Assignee
|
|
US
4940838
- Earliest priority - 24 February 1983
- Filed - 23 February 1984
- Granted - 10 July 1990
- Expected expiry - 10 July 2007
|
Title - Process for the incorporation of foreign DNA into
the genome of dicotyledonous plants
Claim 1
A process for the incorporation of foreign DNA into chromosomes of
dicotyledonous plants, comprising infecting the plants or incubating plant
protoplasts with Agrobacterium bacteria, which contain plasmids, said
Agrobacterium bacteria containing at least one plasmid having the
vir-region of a Ti plasmid but no T-region, and at least one other plasmid
having a T-region with incorporated therein foreign DNA but no vir-region.
|
Claim 13
A process for the production of Agrobacterium bacteria which
contains at least one plasmid which has the vir-region of a Ti-plasmid but no
T-region and at least one other plasmid which has a T-region with foreign DNA
incorporated in it but no vir-region, comprising:
(a) using Escherichia coli as a host and incorporating foreign DNA
into a plasmid therein which contains a T-region and a replicator having a broad
bacterial host range
(b) introducing the resulting plasmid into
Agrobacterium bacteria which contain at least one plasmid which has the
vir-region of a Ti-plasmid but no T-region.
|
The United States patent 4 940 838 claims
- an Agrobacterium having binary vectors where the T-DNA region and
the vir region are located in two different plasmids. The plasmid that
contains the T-region lacks the vir region, and vice versa. The
vir region is fundamental for the transfer of the T-region into the
plant genome.
- the integration of foreign DNA into the genome of a dicot plant. The foreign
DNA is located in the T-region
|
Syngenta Mogen B.V.
|
|
US
5464763
- Earliest priority - 24 February 1983
- Filed - 23 December 1993
- Granted - 7 November 1995
- Expected expiry - 10 July 1997
|
Title - Process for the incorporation of foreign DNA into
the genome of dicotyledonous plants
Claim 1
A process for incorporating into the genome of dicotyledonous plants foreign
DNA, comprising infecting the plants or plant cells or incubating plant
protoplasts with Agrobacterium strains, which contain plasmids, said
Agrobacterium strains containing at least one plasmid having the
vir-region of a Ti plasmid but no T-region, and at least one other plasmid
having a T-region but no vir-region, said T-region being composed of naturally
occurring border sequences consisting of about 23 base pairs at the extremities
of said T-region and only foreign DNA between said border sequences, the
vir-region plasmid and the T-region plasmid containing no homology which could
lead to cointegrate formation.
|
Claim 2
Agrobacterium strains, comprising at least one plasmid having the
vir-region of a Ti plasmid but no T-region, and at least one other plasmid
having a T-region but no vir-region, said T-region being composed of naturally
occurring border sequences consisting of about 23 base pairs at the extremities
of said T-region and only foreign DNA between said sequences, the vir-region
plasmid and the T-region plasmid containing no homology which could lead to
cointegrate formation.
|
Claim 3
A process for the production of Agrobacterium strains comprising at
least one plasmid having the vir-region of a Ti plasmid but no T-region, and at
least one other plasmid having a T-region but no vir-region, said T-region being
composed of naturally occurring border sequences consisting of about 23 base
pairs at the extremities of said T-region and only foreign DNA between said
border sequences, the vir-region plasmid and the T-region plasmid containing no
homology which could lead to cointegrate formation, said process comprising:
(a) incorporating non-Agrobacterium foreign DNA into a plasmid
having a T-region and a replicator having a broad bacterial host range, wherein
said foreign DNA becomes part of the T-region,
(b) cloning the resulting plasmid in Escherichia coli; and
(c) introducing the resulting plasmid into Agrobacterium strains
which contain at least one plasmid which has the vir-region of a Ti-plasmid but
no T-region.
|
Claim 12
A process for incorporating in the genome of dicotyledonous plants foreign
DNA, comprising infecting the plants or plant cells or incubating plant
protoplasts with Agrobacterium strains, which contain plasmids, said
Agrobacterium strains containing at least one plasmid having the
vir-region of a Ti-plasmid but no T-region, and at least one other plasmid
having a single T-region but no vir-region, said single T-region being composed
of naturally occurring border sequences at the extremities of said single
T-region, the vir-region plasmid and the T-region plasmid containing no homology
which could lead to cointegrate formation.
|
Granted US 5464763 is a continuation of US 07/550736 (now
abandoned), which is a continuation of now granted US 4940838
(see above).
The United States patent US 5464763 includes the elements
and methods claims of the United States patent US 4940838 and
additionally claims
- the presence of only foreign DNA between the 23 bp borders of the T-region,
and
- the absence of a region of homology between the plasmid having the T-region
and the plasmid bearing the vir region that might lead to cointegrate
formation. This characteristic is essential to maintain the two plasmids as
individual molecules.
|
|
EP
120516
- Earliest priority - 24 February 1983
- Filed - 21 February 1984
- Granted - 23 October 1991
- Expired - 21 February 2004
|
Title - A process for the incorporation of foreign DNA into
the genome of dicotyledonous plants; Agrobacterium tumefaciens
bacteria and a process for the production thereof
Claim 1
A process for the incorporation of foreign DNA into chromosomes of
dicotyledonous plants comprising infecting the plants or incubating plant
protoplasts with Agrobacterium bacteria, which contain plasmids, said
Agrobacterium bacteria containing at least one plasmid having the
vir-region of a Ti-plasmid but no T-region, and at least one other plasmid
having an artificial T-region with only foreign DNA between the 23 base pairs at
the extremeties of the wild type T-region, but no vir-region, the vir-region
plasmid and the T-region plasmid containing no homology which could lead to
cointegrate formation.
|
Claim 2
Agrobacterium bacteria, comprising at least one plasmid having the
vir-region of a Ti-plasmid but no T- region, and at least one other plasmid
having an artificial T-region with only foreign DNA between the 23 base pairs at
the extremeties of the wild type T-region, but no vir-region, the vir-region
plasmid and the T-region plasmid containing no homology which could lead to
cointegrate formation.
|
Designated States at the time of grant are: Austria (cancelled as reported on
INPADOC), Belgium (expired as reported on INPADOC), Switzerland (ceased as
reported on INPADOC), Germany, France, United Kingdom (expired as reported on
INPADOC), Italy, Liechtenstein, Luxembourg, Netherlands (lapsed as reported on
INPADOC), Sweden
The subject matter claimed in the European patent 120516 B1
is a combination of the claims of the United States patents. In the European
patent, the T-region of one of the binary vectors is claimed as an artificial
T-region, which contains only foreign DNA between the 23 bp T-border sequences.
|
| Remarks |
Related patents granted in Japan (JP 7036751 and JP 7046993, both expired on
23 February 2004 as reported by the Japan Patent Office).
|
Note: Patent information on this page was last updated on 24 February 2006.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
status of patents, and case law may have resulted in changes in the
landscape. CAMBIA makes no warranty that it is correct or up to date at
this time and accepts no liability for any use that might be made of it.
Corrections or updates to the information are welcome. Please send an email to
info@bios.net.
There are no comments.