Patents granted to Leiden University & Schilperoort
Disclosing binary vector systems, the following patents use an
Agrobacterium having either the T-region or the vir region
integrated in the chromosome of the bacterium. The target plants are dicots and
monocot plants from the families Amaryllidaceae (e.g., onion, garlic)
and Liliaceae (e.g., asparagus).
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5149645
- Earliest priority - 4 June 1984
- Filed - 5 December 1989
- Granted - 22 September 1992
- Expected expiry - 4 December 2009
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Title - Process for introducing foreign DNA into the genome
of plants
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Claim 1
Agrobacteria comprising a T-region and a vir-region in their DNA,
the T-region being stably integrated into the bacterial chromosome.
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Claim 2
Agrobacteria comprising a T-region and a vir-region in their DNA,
the vir-region being stably integrated into the bacterial chromosome.
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Claim 3
A process for the transfer of recombinant DNA into the cells or protoplasts
of plants selected from the group consisting of dicotyledonous plants and
monocotyledonous plants of the families Liliaceae and
Amaryllidaceae, said process comprising infecting the plants or plant
cells, or incubating protoplasts from the plants, with Agrobacterium
bacteria which contain in their genetic material a vir-region from the Ti
plasmid of Agrobacterium and at least one T-region, wherein said
T-region comprises said recombinant DNA flanked on both sides by border
sequences as present in wild-type of Agrobacterium, and wherein said
vir-region is integrated into the chromosome of said Agrobacterium
bacteria prior to said infecting or incubating step.
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Claim 5
A process for the transfer of recombinant DNA into the cells or protoplasts
of plants selected from the group consisting of dicotyledonous plants and
monocotyledonous plants of the families Liliaceae and
Amaryllidaceae, said process comprising infecting the plants or plant
cells, or incubating protoplasts from the plants, with Agrobacterium
bacteria which contain in their genetic material a vir-region from the Ti
plasmid of Agrobacterium and at least one T-region, wherein said T-region
comprises said recombinant DNA flanked on both sides by border sequences as
present in wild-type T-region of Agrobacterium, and wherein each of
said T-region is integrated into the chromosome of said Agrobacterium
bacteria prior to said infecting or incubating step.
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The United States patent 5 149 645 claims:
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Agrobacterium having either a T-region or a vir region as
part of the chromosome of the bacterium and further containing a T-region or
vir region, respectively, as part of the Agrobacterium's DNA.
The claims to Agrobacterium don't exclude both the T-region and vir
region being part of the bacterial chromosome.
- a process to transfer recombinant DNA that is part of a T-region in
Agrobacterium into dicot cells and monocot cells of the families
Amaryllidaceae and Liliaceae.
- process claims require that the border sequences flanking the
T-region/recombinant DNA are as present in wild-type Agrobacterium and
so mutated sequences that nevertheless could function as border sequences might
be outside of the scope of the claims in the US.
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Leiden University and Schilperoort
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EP
176112
- Earliest priority - 4 June 1984
- Filed - 3 June 1985
- Granted - 16 May 1990
- Expected expiry - 2 June 2005
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Title - Process for introducing foreign DNA into the genome
of plants
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Claim 1
A process for the incorporation of foreign DNA into the genome of plants by
infecting the plants or by incubating plant protoplasts with
Agrobacterium bacteria, characterized in that Agrobacterium
bacteria are used, which contain in their chromosome at least one T-region or a
vir-region or both, on the understanding that in the first two cases the
bacteria contain a plasmid comprising a vir-region or a T-region resp.
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Claim 4
Agrobacterium bacteria containing in their chromosomes at least one
T-region or a vir-region or both.
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Designated States at the time of grant are: Austria (lapsed as reported by
EPO), Belgium, Switzerland (lapsed as reported by EPO), Germany, France, United
Kingdom (expired as reported by INPADOC), Italy, Liechtenstein (lapsed as
reported by EPO), Luxembourg (lapsed as reported by EPO), Netherlands (lapsed as
reported by INPADOC), Sweden (lapsed as reported by INPADOC)
In the European patent EP 176 112 B1, claims recite:
- either a T-region or a vir region or both integrated into genome of
Agrobacterium;
- A process for transforming plants (any type) - wild-type T border sequences
are not required in the broadest claim.
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Remarks
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Related patent granted in Japan (JP 2006294, term expired as reported by the
Japan Patent Office).
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Note: Patent information on this page was last updated on 24 February 2006.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
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