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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6265638
- Earliest priority - 1 October 1998
- Filed - 28 September 1999
- Granted - 24 July 2001
- Expected expiry - 27 September 2019
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Title - Method of plant transformation
Claim 1
An Agrobacterium strain comprising a helper plasmid and at least two
binary vector plasmids that are stably inherited, wherein each of said binary
vector plasmids comprises at least one T-DNA region comprising a heterologous
nucleotide sequence of interest.
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Claim 12
A method for co-transformation of a plant with at least two unique
heterologous nucleotide sequences of interest, said method comprising the steps
of
a) contacting a tissue from said plant with an Agrobacterium strain
comprising a helper plasmid and at least two binary vector plasmids that are
stably inherited, wherein each of said binary vector plasmids comprises at least
one T-DNA region, wherein each of said T-DNA regions comprises one of said
unique nucleotide sequences, wherein at least one of said nucleotide sequences
comprises at least an expression cassette comprising a plant scorable marker
gene;
b) co-cultivating said tissue with said Agrobacterium;
c) culturing the tissue in a culture medium comprising an antibiotic capable of
inhibiting the growth of Agrobacterium;
d) screening the tissue
for expression of said plants scorable marker gene; and
e) regenerating a
transformed plant from tissue that expresses said plant scorable marker gene.
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The present United States patent claims:
- an Agrobacterium strain having at least two binary vectors with
T-DNA regions containing a heterologous sequence each (which are not necessarily
genes and further, are not necessarily different sequences), and a helper
plasmid; and
- a method to co-transform a plant tissue by co-cultivating the tissue with an
Agrobacterium strain as described above, where at least one of the
heterologous sequences is an expression cassette comprising scorable marker and
is used to screen for the transformed tissue. A transformed plant is regenerated
from the selected transformed tissue.
Without specifying that limits of the heterologous sequences in claim 1,
possible ambiguities arise. For example, how large a sequence does a
heterologous sequence have to be before it falls within the scope of the claim?
A single nucleotide? The claim also could cover scenarios where the two vectors
each have identical heterologous sequences and so be anticipated by art to a
single type of binary plasmid vector including a heterologous sequence provided
with a helper plasmid.
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Pioneer Hi-Bred International
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EP
1117816
- Earliest priority - 1 October 1998
- Filed - 28 September 1999
- Granted - 21 December 2005
- Expected expiry - 27 September 2019
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Title - Method of plant transformation
Claim 1
An Agrobacterium strain comprising a helper plasmid and at least two
binary vector plasmids, wherein each of said binary vector plasmids comprises at
least one T-DNA region comprising a heterologous nucleotide sequence of
interest.
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Claim 12
A method for co-transformation of a plant with at least two unique
heterologous nucleotide sequences of interest, said method comprising the steps
of:
a) contacting a tissue from said plant with an Agrobacterium strain
comprising a helper plasmid and at least two binary vector plasmids, wherein
each of said binary vector plasmids comprises at least one T-DNA region, wherein
each of said T-DNA regions comprises one of said unique nucleotide sequences,
wherein at least one of said nucleotide sequences comprises at least an
expression cassette comprising a plant scorable marker gene;
b)
co-cultivating said tissue with said Agrobacterium;
c) culturing
the tissue in a culture medium comprising an antibiotic capable of inhibiting
the growth of Agrobacterium;
d) screening the tissue for
expression of said plant scorable marker gene; and
e) regenerating a
transformed plant from tissue that expresses said plant scorable marker gene.
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Designated contracting States at the time of grant are: Austria, Belgium,
Switzerland, Cyprus, Germany, Denmark, Spain, Finland, France, United Kingdom,
Greece, Ireland, Italy, Liechtenstein, Luxembourg, Monaco, Netherlands,
Portugal, Sweden.
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AU
764100
- Earliest priority - 1 October 1998
- Filed - 28 September 1999
- Granted - 7 August 2003
- Expected expiry - 27 September 2019
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Title - Method of plant transformation
Claim 1
An Agrobacterium strain comprising a helper plasmid and at least two
binary vector plasmids, wherein each of said binary vector plasmids comprises at
least one T-DNA region comprising a heterologous nucleotide sequence of
interest.
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Claim 12
A method for co-transformation of a plant with at least two unique
heterologous nucleotide sequences of interest, said method comprising the steps
of:
a) contacting a tissue from said plant with an Agrobacterium strain
comprising a helper plasmid and at least two binary vector plasmids, wherein
each of said binary vector plasmids comprises at least one T-DNA region, wherein
each of said T-DNA regions comprises one of said unique nucleotide sequences,
wherein at least one of said nucleotide sequences comprises at least an
expression cassette comprising a plant scorable marker gene;
b)
co-cultivating said tissue with said Agrobacterium;
c) culturing
the tissue in a culture medium comprising an antibiotic capable of inhibiting
the growth of Agrobacterium;
d) screening the tissue for
expression of said plant scorable marker gene; and
e) regenerating a
transformed plant from tissue that expresses said plant scorable marker gene.
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CA 2344700
- Earliest priority - 1 October 1998
- Filed - 28 September 1999
- Granted - 29 March 2005
- Expected expiry - 27 September 2019
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Title - Method of plant transformation
Claim 1
An Agrobacterium strain comprising a helper plasmid and at least two
binary vector plasmids, wherein each of said binary vector plasmids comprises at
least one T-DNA region comprising a heterologous nucleotide sequence of
interest.
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Claim 12
A methid for co-transformation of a plant with at least two unique
heterologous nucleotide sequences of interest, said method comprising the steps
of:
a) contacting a tissue from said plant with an Agrobacterium strain
comprising a helper plasmid and at least two binary vector plasmids, wherein
each of said binary vector plasmids comprises at least one T-DNA region, wherein
each of said T-DNA regions comprises one of said unique nucleotide sequences,
wherein at least one of said nucleotide sequences comprises at least an
expression cassette comprising a plant scorable marker gene;
b)
co-cultivating said tissue with said Agrobacterium;
c) culturing
the tissue in a culture medium comprising an antibiotic capable of inhibiting
the growth of Agrobacterium;
d) screening the tissue for
expression of said plants scorable marker gene; and
e) regenerating a
transformed plant from tissue that expresses said plant scorable marker gene.
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WO
2000/18939
- Earliest priority - 1 October 1998
- Filed - 28 September 1999
- OPI - 14 September 2000
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Title - Method of plant transformation
Claim 1
An Agrobacterium strain comprising a helper plasmid and at least two
binary vector plasmids that are stably inherited, wherein each of said binary
vector plasmids comprises at least one T-DNA region comprising a heterologous
nucleotide sequence of interest.
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Claim 12
A method for co-transformation of a plant with at least two unique
heterologous nucleotide sequences of interest, said method comprising the steps
of:
a) contacting a tissue from said plant with an Agrobacterium strain
comprising a helper plasmid and at least two binary vector plasmids that are
stably inherited, wherein each of said binary vector plasmids comprises at least
one T-DNA region, wherein each of said T-DNA regions comprises one of said
unique nucleotide sequences, wherein at least one of said nucleotide sequences
comprises at least an expression cassette comprising a plant scorable marker
gene;
b) co-cultivating said tissue with said Agrobacterium;
c) culturing the tissue in a culture medium comprising an antibiotic capable of
inhibiting the growth of Agrobacterium;
d) screening the tissue
for expression of said plants scorable marker gene; and
e) regenerating a
transformed plant from tissue that expresses said plant scorable marker gene.
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