Turfgrass

Summary

Rutgers University has filed a PCT application directed to transformation of turfgrass callus with Agrobacterium. The bacterium contains a vector with a selectable marker gene and an insertion site for any coding sequence, both of them controlled by promoters isolated from monocot plants. The promoters, according to the applicants, function efficiently in turfgrass cells.

Specific Patent Information

Patent Number

Title, Independent Claims and Summary of Claims

Assignee

EP 1100876 A1

Earliest priority - 17 July 1998
Filed - 13 July 1999
Application pending

Title - Agrobacterium-mediated transformation of turfgrass

Claim 1

A method of producing a transgenic turfgrass plant, comprising the steps of: A) providing regenerable callus tissue from the turfgrass plant;
B) inoculating the tissue with Agrobacterium carrying at least one vector for transformation, the vector comprising virulence genes that confer strong infectivity to Agrobacterium, in which vector is inserted a heterologous DNA construct operably linked to a promoter from a monocotyledonous species, and a selectable marker gene conferring antibiotic resistance to transformed cells operably linked to a promoter from a monocotyledonous species;
C) culturing the inoculated tissue under conditions that enable the Agrobacterium vector to transform cells of the tissue;
D) selectively culturing the inoculated tissue on a selection medium comprising the antibiotic; and
E) regenerating a transformed turfgrass plant from the selectively cultured tissue.

Claim 11

A superbinary vector system for Agrobacterium-mediated transformation of turfgrass, which comprises:

A) a virulence region from a Ti plasmid of an A. tumefaciens strain that confers to the strain as strong a virulence as that displayed by A. tumefaciens strain 281;
B) a selectable marker gene operably linked to a promoter obtained from a gene of a monocotyledonous plant; and
C) a site for insertion of at least one additional coding sequence, operably linked to a promoter obtained from a gene of a monocotyledonous plant, the promoter being the same as or different from the promoter operably linked to the selectable marker gene.

A method of producing a transgenic turfgrass plant by inoculating a regenerable turfgrass callus with Agrobacterium containing an antibiotic resistance linked to a monocot promoter. The antibiotic resistance is used as a selectable marker for the transformed tissue. A transformed turfgrass plant is regenerated from the selected transformed tissue.

A super-binary vector used for the Agrobacterium-mediated transformation of turfgrass comprising: a virulence region, a selectable marker linked to a monocot promoter, and an insertion site for a coding sequence. This site is linked to the same or a different monocot promoter.

Rutgers University

Remarks

National phase entry of WO 2000/04133 in Australia (AU 52136/99 A) has lapsed.

Note: Patent information on this page was last updated on 9 February 2006.

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