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Banana (Musa) - Specific Patent Information - part 2

Patent Number

Title, Independent Claims and Summary of Claims

Assignee

US 6133035

  • Earliest priority - 16 July 1997
  • Filed - 16 July 1997
  • Granted - 17 October 2000
  • Expected expiry - 15 July 2017

Title - Method of Genetically Transforming Banana Plants

Claim 1

A method of producing a transformed banana plant comprising transforming banana embryogenic material from inflorescences with Agrobacterium containing a gene of interest and regenerating a transformed banana plant from the transformed embryogenic material.

Claim 7

A method of producing a transformed banana plant comprising transforming a banana somatic embryo from inflorescences with Agrobacterium containing a gene of interest and regenerating a transformed banana plant from the transformed somatic embryo.

Claim 11

A method of genetically transforming banana, the method comprising: A) culturing somatic banana plant tissue from inflorescences in a medium to obtain at least one somatic embryo structure or pro-embryo structure;
B) culturing the somatic embryo or pro-embryo structure in a medium to obtain embryogenic material;
C) transforming the embryogenic material with Agrobacterium cells having at least one exogenous DNA sequence to produce transformed embryogenic material;
D) culturing the transformed embryogenic material in a medium to produce at least one transformed somatic embryo; and
E) germinating the transformed somatic embryo in a medium to produce a mature plantlet capable of being transferred to soil conditions.

Claim 19

A method of genetically transforming banana, the method comprising:
A) culturing somatic banana plant tissue from inflorescences in a medium to obtain at least one somatic embryo structure or pro-embryo structure;
B) culturing the somatic embryo or pro-embryo structure in a medium to obtain embryogenic material;
C) culturing the embryogenic material in a medium to produce at least one somatic embryo;
D) transforming the somatic embryo produced in step (c) with Agrobacterium cells having at least one exogenous DNA sequence to produce transformed somatic embryos;
E) multiplying the transformed somatic embryo to produce additional transformed somatic embryos; and
F) germinating the transformed somatic embryo to produce a mature plantlet capable of being transferred to soil conditions.

Methods for transformation of embryogenic material from banana inflorescences with Agrobacterium having a gene of interest. Production of plantlets capable of being transferred to soil conditions.

Zeneca & DNA Plant
Technology Corp.

(Zeneca is now Syngenta)

AU 744496 B2

  • Earliest priority - 16 July 1997
  • Filed - 13 July 1998
  • Granted - 28 February 2002
  • Expected expiry - 12 July 2018

Title - Method of Genetically Transforming Banana Plants

Claim 1

A method of producing a transformed banana plant comprising transforming banana embryogenic material with Agrobacterium containing a gene of interest and regenerating a transformed banana plant from the transformed embryogenic material.

Claim 7

A method of producing a transformed banana plant comprising transforming a banana somatic embryo with Agrobacterium containing a gene of interest and regenerating a transformed banana plant from the transformed somatic embryo.

Claim 11

A method of genetically transforming banana, the method comprising:
A) culturing somatic banana plant tissue in a medium to obtain at least one somatic embryo structure or pro-embryo structure;
B) culturing the somatic embryo or pro-embryo structure in a medium to obtain embryogenic material;
C) transforming the embryogenic material with Agrobacterium cells having at least one exogenous DNA sequence to produce transformed embryogenic material;
D) culturing the transformed embryogenic material in a medium to produce at least one transformed somatic embryo; and
E) germinating the transformed somatic embryo in a medium to produce a mature plantlet capable of being transferred to soil conditions.

Claim 19

A method of genetically transforming banana, the method comprising:
A) culturing somatic banana plant tissue in a medium to obtain at least one somatic embryo structure or pro-embryo structure;
B) culturing the somatic embryo or pro-embryo structure in a medium to obtain embryogenic material;
C) culturing the embryogenic material in a medium to produce at least one somatic embryo;
D) transforming the somatic embryo produced in step (c) with Agrobacterium cells having at least one exogenous DNA sequence to produce transformed somatic embryos;
E) multiplying the transformed somatic embryo to produce additional transformed somatic embryos; and
F) germinating the transformed somatic embryo to produce a mature plantlet capable of being transferred to soil conditions.

The invention claimed in Australia is similar to the related United States patent but the embryogenic material is not from a specific part of a banana plant.

Syngenta Ltd. & DNA Plant Technology Corp.

EP 996329 A1

  • Earliest priority - 16 July 1997
  • Filed - 13 July 1998
  • Application pending

Title - Method of Genetically Transforming Banana Plants

Claim 1

A method of producing a transformed banana plant comprising transforming banana embryogenic material with Agrobacterium containing a gene of interest and regenerating a transformed banana plant from the transformed embryogenic material.

Claim 7

A method of producing a transformed banana plant comprising transforming a banana somatic embryo with Agrobacterium containing a gene of interest and regenerating a transformed banana plant from the transformed somatic embryo.

Claim 11

A method of genetically transforming banana, the method comprising:

A) culturing somatic banana plant tissue in a medium to obtain at least one somatic embryo structure or pro-embryo structure;
B) culturing the somatic embryo or pro-embryo structure in a medium to obtain embryogenic material;
C) transforming the embryogenic material with Agrobacterium cells having at least one exogenous DNA sequence to produced transformed embryogenic material;
D) culturing the transformed embryogenic material in a medium to produce at least one transformed somatic embryo; and
E) germinating the transformed somatic embryo in a medium to produce a mature plantlet capable of being transferred to soil conditions.

Claim 19

A method of genetically transforming banana, the method comprising:

A) culturing somatic banana plant tissue in a medium to obtain at least one somatic embryo structure or pro-embryo structure;
B) culturing the somatic embryo or pro-embryo structure in a medium to obtain embryogenic material;
C) culturing the embryogenic material in a medium to produce at least one somatic embryo;
D) transforming the somatic embryo produced in step (C) with
Agrobacterium cells having at least one exogenous DNA sequence to produce transformed somatic embryos;
E) multiplying the transformed somatic embryo to produce additional transformed somatic embryos; and
F) germinating the transformed somatic embryo to produce a mature plantlet capable of being transferred to soil conditions.

The claims as filed in the European application recite the same as the claims granted in the United States patent US 6133035 with the exception of claim 7, where a banana somatic embryo is not limited to an embryo derived from an inflorescence.

Transformation of somatic embryos of banana with Agrobacterium having a gene of interest. Multiplication of transformed somatic embryos and production of plantlets capable of being transferred to soil conditions.

Remarks

National phase entry of WO 1999/03327 in Japan (JP 2001510021 T2) is still pending.

Note: Patent information on this page was last updated on 14 February 2006.

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