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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6040498
- Earliest priority - 11 August 1998
- Filed - 11 August 1998
- Granted - 21 March 2000
- Expected expiry - 10 August 2018
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Title - Genetically Engineered Duckweed
| Claim 1
A method for stably transforming duckweed tissue with a nucleotide sequence
of interest, the method comprising the steps of:
A) inoculating a duckweed plant tissue with
Agrobacterium comprising a vector which comprises a nucleotide sequence
of interest; and
B) co-cultivating the tissue with the
Agrobacterium to produce stably transformed tissue.
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| Claim 20
A stably transformed duckweed plant comprising a heterologous nucleic acid of
interest incorporated in its genome wherein said plant is produced via an
Agrobacterium-mediated method.
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| Claim 30
A method of producing recombinant proteins or peptides, comprising the steps
of:
A) culturing a stably transformed duckweed plant that expresses at least one
heterologous protein or peptide; and
B) collecting the at least one
heterologous protein or peptide from the duckweed cultures.
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| Claim 48
A method for stably transforming duckweed tissue from the genus
Lemna with a nucleotide sequence of interest, the method comprising the
steps of:
A) inoculating a duckweed plant tissue with Agrobacterium
comprising a vector which comprises a nucleotide sequence of interest, wherein
the duckweed plant tissue is from the genus Lemna; and
B)
co-cultivating the tissue with the Agrobacterium to produce stably
transformed tissue.
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| Claim 59
A stably transformed duckweed plant from the genus Lemna comprising
a heterologous nucleic acid sequence incorporated in its genome wherein said
plant is produced via an Agrobacterium -mediated method.
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| Claim 62
A method of producing recombinant proteins or peptides, comprising:
A) culturing a stably transformed duckweed plant from the genus
Lemna that expresses at least one heterologous protein or peptide; and
B) collecting the at least one protein or peptide from the duckweed
cultures.
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A method for transforming a duckweed tissue by co-cultivating the tissue with
Agrobacterium having a sequence of interest. A method for producing
recombinant proteins or peptides by culturing transformed duckweed plants
expressing the proteins or peptides and collecting them form the cultured
plants.
The same methods are used to transform a duckweed from the genus
Lemna.
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North Carolina State University
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AU
775632 B2
- Earliest priority - 12 August 1997
- Filed - 11 August 1998
- Granted - 19 December 2002
- Expected expiry - 10 August 2018
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Title - Genetically engineered duckweed
Claim 1
A method for producing a stably transformed duckweed plant comprising a
heterologous nucleotide sequence of interest, the method comprising the steps
of:
(a) inoculating a duckweed callus tissue
with an Agrobacterium comprising a vector which is not a super-virulent
vector and which comprises a heterologous nucleotide sequence of interest,
wherein the nucleotide sequence comprises at least one expression cassette
comprising a gene which confers resistance to a selection agent;
(b)
co-cultivating the callus tissue with the
Agrobacterium to produce stably transformed callus tissue; and
(c) regenerating a stably transformed duckweed plant from the
stably transformed callus tissue.
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Claim 40
A method for producing a stably transformed duckweed plant comprising a
chimeric nucleotide sequence of interest, the method comprising the steps of:
(a) inoculating a duckweed callus tissue with an Agrobacterium
comprising a vector which is not a super-virulent vector and which comprises a
chimeric nucleotide sequence of interest, said chimeric nucleotide sequence
comprising a coding sequence operably linked to a transcription initiation
region that is heterologous to said coding sequence;
(b) co-cultivating the
callus tissue with the Agrobacterium to produce stably transformed
callus tissue; and
(c) regenerating a stably transformed duckweed plant
from the stably transformed callus tissue.
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Granted AU 775632 recites a method to produce stably
transformed duckweed with duckweed callus tissue with a ('heterologous' or
'chimeric') nucleotide sequence of interest, which comprises
- inoculation of Agrobacterium,
- co-cultivation of Agrobacterium with the callus tissue, and
- regeneration of transformed callus tissue.
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| Remarks |
- Continuations of the divisional patent application of US 6040498
(US
2004/73968 A1 and
US
2003/115640 A1) are still pending.
- National phase entry of
WO
1999/07210 in Canada (CA 2288895), China (CN 1272762), Europe (EP 1037523),
Japan (JP 2001/513325) are pending.
- Other national phase entry of
WO
1999/07210 includes Israel (IL 132580).
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EP
1021552 A1
- Earliest priority - 10 October 1997
- Filed - 8 October 1998
- Application pending
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Title - Transgenic Lemnaceae
| Claim 1
A genetically stable, transformed Lemnaceae plant and progeny thereof.
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| Claim 12*
A method for the stable genetic transformation of Lemnaceae plants which
comprises:
incubating Lemnaceae plants and/or tissue with
Agrobacterium cells containing a transforming DNA molecule, whereby
cells in said plant tissue become stably transformed with said DNA.
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| Claim 19*
A method for the genetic transformation of a plant comprising: A) cutting the
plant into particles of a size such that they still contain undamaged
meristematic tissue capable of developing into full plants;
B) incubating
said particles with Agrobacterium cells containing transforming DNA
molecules, whereby said transforming DNA is introduced into meristematic cells
in said particles; and
C) producing transformed plants from the transformed
meristematic tissue.
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| Claim 23*
A method for the stable genetic transformation of a Lemnaceae plant
comprising
microinjecting Agrobacterium cells containing a
transforming DNA into the meristematic zone of the plant, whereby the meristemic
tissue becomes stably transformed with said DNA.
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| Claim 25
A) method for the in planta transformation of Lemnaceae plants
comprising: A) exposing the plant's meristematic zone by removal of the daughter
fronds;
B) incubating the plant with Agrobacterium cells capable
of targeting to the meristemic tissue.
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| Claim 37*
A booster medium for enhancing Agrobacterium cell's virulence
comprising plant tissue culture at a pH below about 5.2.
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| Claim 43*
A booster medium for enhancing Agrobacterium cell's virulence
comprising an extract from Lemnaceae plants.
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| Claim 45
A method for maintaining morphogenetic Lemnaceae calli for long-periods of
time comprising culturing the calli in a medium having a low level of sucrose.
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| Claim 47
A method for the regeneration of plants from calli wherein the plant's growth
medium has sucrose levels below 1.5% and comprises: B5, minerals and organic
compounds.
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| Claim 48
A method for the production of highly regenerative calli, wherein the calli's
growth medium has sucrose levels below 1.5% and comprises B5, minerals and
organic compounds.
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| Claim 50
A method for the production of highly regenerative calli, wherein the calli's
growth medium has sucrose levels below 1.5% and comprises B5, minerals, organic
compounds and selection agents.
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| Claim 52
A method for the production of stable transformed plants, wherein the growth
media has sucrose levels below 1.5% and comprises B5, minerals and organic
compounds.
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* Claims directly related to Agrobacterium -mediated transformation
of a Lemnaceae plant.
Different methods for stable genetic transformation of a Lemnaceae plant with
Agrobacterium containing a gene of interest. The methods include
incubating the plants with Agrobacterium, incubating meristematic
tissue with the bacterium, injecting the bacterium into the meristematic tissue
and in planta transformation. The invention also includes methods for
enhancing the virulence of Agrobacterium and for regenerating calli and
transformed plants.
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Yeda Research & Development Co.
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AU
759570 B2
- Earliest priority - 10 October 1997
- Filed - 8 October 1998
- Granted - 17 April 2003
- Expected expiry - 7 October 2018
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Title - Transgenic Lemnaceae
Claim 1
A stably transformed Lemnaceae plant, tissues, products and progeny thereof
of the genus Spirodela, when produced by Agrobacterium-mediated
transformation.
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| Claim 2
A method for the stable genetic transformation of Lemnaceae plants,
comprising the step of:
Incubating meristematic tissues of Lemnaceae with
Agrobacterium cells containing a transforming DNA molecule, wherein the
Agrobacterium cells are A. tumefaciens strains EHA105, EHA 101
or GVE3103, whereby cells in said tissue become stably transformed with said
DNA, and regenerating Lemnaceae from the meristematic tissue.
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Claim 3
A method for the stable genetic transformation of Lemnaceae plants,
comprising the step of:
microinjecting into a meristematic zone of
Lemnaceae plants and/or tissue Agrobacterium cells containing a
transforming DNA molecule, whereby cells in said plant tissue become stably
transformed with said DNA.
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Claim 4
A method for the stable genetic transformation of a Lemnaceae plant,
comprising the steps of:
a) cutting the plant into particles of a size such
that they still contain undamaged meristematic tissue capable of developing into
full plants;
b) incubating said particles with Agrobacterium
cells containing transforming DNA molecules, whereby said transforming DNA is
introduced into meristematic cells in said particles; and
c) producing
transformed plants form the meristematic tissue.
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| Claim 7
A method for the stable genetic transformation of a Lemnaceae plant,
comprising the step of:
microinjecting Agrobacterium cells
containing a transforming DNA molecule into the meristematic zone of the plant,
whereby the meristematic tissue becomes stably transformed with said DNA.
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Claim 8
A method for the in planta transformation of Lemnaceae plants, comprising the
steps of:
a) exposing the plant’s meristematic zone by removal of daughter
fronds; and
b) incubating the plant with Agrobacterium cells
capable of targeting to the meristematic tissue.
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This granted patent claims any Lemnaceae plant, part or whole, that is
transformed using Agrobacterium.
It also claims a method of Agrobacterium-mediated transformation
using the meristematic tissue of Lemnaceae plants.
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| Remarks |
- National phase entry of
WO
1999/19497 in Canada (CA 2312008) is still pending.
- Other national phase entry of
WO
1999/19497 includes Israel (IL 135543).
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