Maize - Specific Patent Information - part 2
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6420630 B1
- Earliest priority - 1 December 1998
- Filed - 1 December 1998
- Granted - 16 July 2002
- Expected expiry - 30 November 2018
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Title - Methods for tissue culturing and transforming elite
inbreds of Zea mays L.
| Claim 1
A method for transforming a line of corn using Agrobacterium
comprising the steps of:
(a) initiating co-cultivation of an immature
embryo from said line with Agrobacterium capable of transferring at
least one gene to tissue of said line to produce an infected embryo;
(b)
applying heat shock treatment during said co-cultivation;
(c) culturing the
infected embryo to initiate callus on a medium comprising an antibiotic;
(d) culturing the resulting callus tissue on a medium comprising a selective
agent;
(e) selecting transformed callus tissue comprising growing Type II
callus; and
(f) regenerating transgenic plants from said Type II callus.
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A method of transforming maize by co-cultivating Agrobacterium
carrying a gene of interest with immature embryos. Heat shock is applied to
enhance DNA integration.
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Stine Biotechnology
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US
6919494 B2
- Earliest priority - 1 December 1998
- Filed - 30 July 2001
- Granted - 19 July 2005
- Expected expiry - 29 July 2021
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Title - Methods for tissue culturing and transforming elite
inbreds of Zea mays L.
| Claim 1
A method for producing a corn plant comprising the steps of:
(a) co-cultivating an immature embryo from said tissue at a temperature of
about 18˚C. to 20˚C. with Agrobacterium capable of transferring at
least one DNA sequence of interest to said tissue to produce an infected embryo;
(b) culturing the infected embryo on a medium comprising an antibiotic to
produce a resulting tissue;
(c) culturing said resulting tissue on a medium comprising a selective agent
and an antibiotic;
(d) selecting transformed tissue having Type II callus; and
(e) regenerating transgenic plants from said Type II callus.
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| Claim 3
A method for transforming a line of corn comprising the steps of:
(a) co-cultivating an immature embryo from said line with
Agrobacterium capable of transferring at least one DNA sequence of
interest to said tissue of said line to produce an infected embryo;
(b) culturing the infected embryo to initiate callus on a medium comprising
an antibiotic and a compound selected from the group consisting of glucose,
maltose, lactose, sorbitol and mannitol, wherein the concentration of said
compound is from 5 g/L to 30 g/L;
(c) culturing the resulting callus tissue on a medium comprising a selective
agent and an antibiotic;
(d) selecting transformed callus tissue comprising growing Type II callus;
and
(e) regenerating transgenic plants from said growing Type II callus.
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| Claim 4
A method for producing a transformed corn plant using Agrobacterium
comprising the steps of:
(a) initiating co-cultivation of an immature embryo from said tissue with
Agrobacterium capable of transferring at least one DNA sequence of
interest to said tissue to produce an infected embryo;
(b) applying heat shock treatment during said co-cultivation;
(c) culturing the infected embryo to initiate callus on a medium comprising
an antibiotic and glucose;
(d) culturing the resulting callus tissue on a medium comprising a selective
agent and an antibiotic;
(e) selecting transformed callus tissue having Type II callus; and
(f) regenerating transgenic plants from said Type II callus.
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Granted patent of application
US
2002/0104131 (see below).
Continuation of US Patent Application 09/203,679 (now
US
Patent 6420630 B1)
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US
2002/0104131 A1
- Earliest priority - 1 December 1998
- Filed - 30 July 2001
- Granted as US 6919494 (see above)
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Title - Methods for tissue culturing and transforming elite
inbreds of Zea mays L.
| Claim 1 (not granted in US 6919494)
A method for stimulating a high frequency production of Type II callus from
immature embryos of elite corn inbreds which comprises culturing said embryos on
a solid medium comprising sucrose and a monosaccharide sugar, wherein the
concentration of said monosaccharide sugar is between about 5 g/L and about 30
g/L.
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Claim 4
A method for transforming elite lines of corn using Agrobacterium
comprising the steps of:
(a) co-cultivating an immature embryo from said elite line with
Agrobacterium capable of transferring at least one gene to tissue of
said elite line on a solid medium to produce an infected embryo;
(b) culturing the infected embryo on a solid medium comprising an antibiotic;
(c) culturing the resulting tissue on a solid medium comprising a selective
agent to select for transformed tissue;
(d) selecting transformed tissue with growing Type II callus capable of
forming water tower embryo structures; and
(e) regenerating plants from said embryo structures.
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Claim 22
A method for transforming elite lines of corn using Agrobacterium
comprising the steps of:
(a) co-cultivating an immature embryo from said elite line with
Agrobacterium capable of transferring at least one gene to tissue of
said elite line on a solid medium to produce an infected embryo;
(b) culturing the infected embryo on a solid medium comprising an antibiotic
and a monosaccharide sugar in an amount of from 5 g/L to 30g/L;
(c) culturing the resulting tissue on a solid medium comprising an antibiotic
and a selective agent;
(d) culturing the resulting tissue on a solid medium comprising a selective
agent to select for transformed tissue;
(e) selecting transformed tissue with growing Type II callus capable of
forming water tower embryo structures; and
(f) regenerating plants from said embryo structures.
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Claim 32
A method for transforming elite lines of corn using Agrobacterium
comprising the steps of:
(a) co-cultivating at a temperature of 19[deg.] C. an immature embryo from
said elite line with Agrobacterium capable of transferring at least one
gene to tissue of said elite line on a solid medium to produce an infected
embryo, said Agrobacterium is selected from Agrobacterium one
to two days after rescue from frozen glycerol stocks;
(b) culturing the infected embryo on a solid medium comprising an antibiotic
at a concentration of 15 mg/L to 75 mg/L and a monosaccharide sugar selected
from the group consisting of glucose, maltose, lactose, sorbitol and mannitol in
an amount of from 5 g/L to 30 g/L;
(c) culturing the resulting tissue on a solid medium comprising an antibiotic
and a selective agent;
(d) culturing the resulting tissue on a solid medium comprising a selective
agent to select for transformed tissue;
(e) selecting transformed tissue with growing Type II callus capable of
forming water tower embryo structures; and
(f) regenerating plants from said embryo structures.
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A method in which formation of Type II callus (a preferred form of target
tissue for the transformation with Agrobacterium) from immature embryos
is enhanced by adding sucrose and a monosaccharide to the medium.
Continuation of US Patent Application 09/203,679 (now US Patent US
6420630 B1)
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US
2002/0104132 A1
- Earliest priority - 1 December 1998
- Filed - 30 July 2001
- Abandoned - 19 September 2005
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Title - Methods for tissue culturing and transforming elite
inbreds of Zea mays L.
| Claim 1
A method for stimulating a high frequency production of Type II callus from
immature embryos of elite corn inbreds which comprises
- culturing said embryos on a solid medium comprising sucrose and a
monosaccharide sugar, wherein the concentration of said monosaccharide sugar is
between about 5 g/L and about 30 g/L.
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| Claim 4
A method for transforming elite lines of corn using Agrobacterium
comprising the steps of:
(a) co-cultivating an immature embryo from said elite line with
Agrobacterium capable of transferring at least one gene to tissue of
said elite line on a solid medium to produce an infected embryo;
(b) culturing the infected embryo on a solid medium comprising an antibiotic;
(c) culturing the resulting tissue on a solid medium comprising a selective
agent to select for transformed tissue;
(d) selecting transformed tissue with growing Type II callus capable of
forming water tower embryo structures; and
(e) regenerating plants from said embryo structures.
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| Claim 22
A method for transforming elite lines of corn using Agrobacterium
comprising the steps of:
(a) co-cultivating an immature embryo from said elite line with Agrobacterium
capable of transferring at least one gene to tissue of said elite line on a
solid medium to produce an infected embryo;
(b) culturing the infected embryo on a solid medium comprising an antibiotic
and a monosaccharide sugar in an amount of from 5 g/L to 30g/L;
(c) culturing the resulting tissue on a solid medium comprising an antibiotic
and a selective agent;
(d) culturing the resul ting tissue on a solid medium comprising a selective
agent to select for transformed tissue;
(e) selecting transformed tissue with growing Type II callus capable of
forming water tower embryo structures; and
(f) regenerating plants from said embryo structures.
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| Claim 32
A method for transforming elite lines of corn using Agrobacterium
comprising the steps of:
(a) co-cultivating at a temperature of 19[deg.] C. an immature embryo from
said elite line with Agrobacterium capable of transferring at least one
gene to tissue of said elite line on a solid medium to produce an infected
embryo, said Agrobacterium is selected from Agrobacterium one
to two days after rescue from frozen glycerol stocks.;
(b) culturing the infected embryo on a solid medium comprising an antibiotic
at a concentration of 15 mg/L to 75 mg/L and a monosaccharide sugar selected
from the group consisting of glucose, maltose, lactose, sorbitol and mannitol in
an amount of from 5 g/L to 30 g/L;
(c) culturing the resulting tissue on a solid medium comprising an antibiotic
and a selective agent;
(d) culturing the resulting tissue on a solid medium comprising a selective
agent to select for transformed tissue;
(e) selecting transformed tissue with growing Type II callus capable of
forming water tower embryo structures; and
(f) regenerating plants from said embryo structures.
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A method as in the application above but specifically designed for elite corn
inbred lines.
Divisional application from US Patent Application 09/203,679 (now US Patent
US 6420630 B1), this application has been abandoned due to
failure to respond to a USPTO action according to the USPTO.
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US
2005/0278802 A1
- Earliest priority - 1 December 1998
- Filed - 18 July 2005
- Application pending
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Title - Methods for tissue culturing and transforming elite inbreds of
Zea mays L.
| Claim 35
A method for producing a corn plant comprising the steps of:
(a) culturing a corn embryo on a medium comprising a compound selected from
the group consisting of glucose, maltose, lactose, sorbitol, and mannitol,
wherein said compound is in an amount of from about 5 g/L to 30 g/L, to produce
a type II callus; and
(b) regenerating a plant.
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This is a divisional application of now granted US 6420630.
All of the independent claims preceeding claim 35 has been cancelled.
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Note: Patent information on this page was last updated on 7 February 2006.
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time it was collated. New patents and patent applications, altered
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