Specific Patent Onions - Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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WO
2000/44919 A1
- Earliest priority - 29 January 1999
- Filed - 10 December 1999
- OPI - 3 August 2000
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Title - Transformation and Regeneration of Allium
plants
A preferred embodiment utilizes the alliinase gene, which encodes an
enzyme involved in sulfur metabolism.
| Claim 1
A method of transforming plants of the Allium genus.
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| Claim 3
A method of transforming plants of the Allium genus comprising
inoculating an embryo culture of an Allium species with an
Agrobacterium tumefaciens strain containing a suitable vector or
plasmid.
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| Claim 9
A method of transforming Allium using immature embryos as an explant
source, including:
(a) isolating immature embryos of the Allium
plant to be transformed;
(b) innoculating cultures of the immature embryos
with an Agrobacterium tumefaciens strain containing a binary
vector;
(c) wounding embryos and infiltrating embryos with
agrobacteria;
(d) transferring embryos to a selective medium;
(e)
culturing embryo pieces;
(f) selecting putative transgenic cultures;
and
(g) regenerating plants.
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New Zealand Institute for Crop & Food Research Ltd.
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AU
763531 C
- Earliest priority - 29 January 1999
- Filed - 10 December 1999
- Granted - 24 July 2003
- Amended - 26 February 2004
- Expected expiry - 9 December 2019
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Title - Transformation and regeneration of Allium
plants
| Claim 1
A method of transforming plants of the Allium genus comprising the
following steps:
(a) delivering previously manipulated DNA into embryo, or
embryo derived culture cell types of the Allium genus via vector or
direct gene transfer;
(b) selecting transformed plant material;
(c)
culturing and regenerating the transformed plants;
wherein the
transformation is carried out without passage through a callus phase.
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| Claim 7
A method of transforming Allium using immature embryos as an explant
source, including:
(a) isolating immature embryos of the Allium
plant to be transformed;
(b) innoculating cultures of the immature embryos
with an Agrobacterium tumefaciens strain containing a binary
vector;
(c) wounding embryos and infiltrating embryos with
agrobacteria;
(d) transferring embryos to a selective medium;
(e)
culturing embryo pieces;
(f) selecting putative transgenic cultures;
and
(g) regenerating plants.
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| Remarks |
- Related patent granted in New Zealand (NZ 513184).
- National phase entries of WO 2000/44919 in Canada (CA 2361143 A1) and Europe
(EP 1144664 A1) are still pending.
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EP
1180927 B1
- Earliest priority - 5 May 1999
- Filed - 5 May 2000
- Granted - 21 December 2005
- Expected expiry - 4 May 2020
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Title - Transformation of Allium sp with
Agrobacterium using embrogenic callus cultures
A method of transforming Allium species (onions) with
Agrobacterium carrying a heterologous gene. A preferred embodiment
utilizes immature embryos and flower buds as target tissue for transformation.
| Claim 1
A method for transforming an Allium species with a heterologous
gene, the method comprising the step of: contacting embryogenic callus material
from an Allium species with a bacterium belonging to the genus
Agrobacterium which contains a heterologous gene.
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| Claim 8
A method for transforming an Allium species with a heterologous
gene, the method comprising the steps of:
a. culturing immature embryos or
flower buds from an Allium species on an initiation medium for a period
of from about 2 to about 6 months until embryogenic callus material forms on the
embryos or flower buds;
b. transferring the embryogenic callus material to
a coculture medium and contacting the embryogenic callus material with a
suspension of Agrobacterium rhizogenes or Agrobacterium
tumefaciens containing a heterologous gene;
c. incubating the
embryogenic callus material with the Agrobacterium rhizogenes or
Agrobacterium tumefaciens for a period of from about 2 to about 4 days;
and
d. removing the Agrobacterium rhizogenes or Agrobacterium
tumefaciens from the transformed embryogenic callus material.
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Designated contracting State at the time of grant is Spain.
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Seminis Vegetable Seeds Inc. (US)
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EP 1180927 A1
- Earliest priority - 5 May 1999
- Filed - 5 May 2000
- Granted as EP 1180927 B1 (see above)
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Title - Transformation of Allium sp with
Agrobacterium using embrogenic callus cultures
A method of transforming Allium species (onions) with
Agrobacterium carrying a heterologous gene. A preferred embodiment
utilizes immature embryos and flower buds as target tissue for transformation.
| Claim 1 (granted)
A method for transforming an Allium species with a heterologous
gene, the method comprising the step of: contacting embryogenic callus material
from an Allium species with a bacterium belonging to the genus
Agrobacterium which contains a heterologous gene.
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Claim 9 (granted)
A method for transforming an Allium species with a heterologous
gene, the method comprising the steps of:
a. culturing immature embryos or flower buds from an Allium species
on an initiation medium for a period of from about 2 to about 6 months until
embryogenic callus material forms on the embryos or flower buds;
b.
transferring the embryogenic callus material to a coculture medium and
contacting the embryogenic callus material with a suspension of Agrobacterium
rhizogenes or Agrobacterium tumefaciens containing a heterologous gene;
c. incubating the embryogenic callus material with the Agrobacterium
rhizogenes or Agrobacterium tumefaciens for a period of from about
2 to about 4 days; and
d. removing the Agrobacterium rhizogenes
or Agrobacterium tumefaciens from the transformed embryogenic callus
material.
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AU
780954 B2
- Earliest priority - 5 May 1999
- Filed - 5 May 2000
- Granted - 28 April 2005
- Expected expiry - 4 May 2020
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Title - Transformation of Allium sp. with
Agrobacterium using embryogenic callus cultures
Claims of this patent are limited to transforming Allium cepa and
A. fistulosum using Agrobacterium.
| Claim 1
A method for transforming an Allium cepa or Allium
fistulosum with a DNA of interest from a heterologous gene, the method
comprising the steps of: contacting embryogenic callus material from an
Allium cepa or Allium fistulosum with a bacterium
belonging to the genus Agrobacterium which contains a DNA of interest
from a heterologous gene and obtaining a transformed Allium cepa or
Allium fistulosum embryogenic callus under selective conditions.
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| Claim 8
A method for transforming a plant or plant tissue of an Allium
cepa or Allium fistulosum with a DNA of interest from a
heterologous gene, the method comprising the steps of:
a. culturing
immature embryos or flower buds from an Allium cepa or
Allium fistulosum on an initiation medium for a period of from about 2
to about 6 months until an embryogenic callus forms on the embryos or flower
buds;
b. transferring the embryogenic callus to a coculture medium and
contacting the embryogenic callus with a suspension of Agrobacterium
rhizogenes or Agrobacterium tumefaciens containing a DNA of
interest from a heterologous gene; and
c. obtaining a transformed
Allium cepa or Allium fistulosum embryogenic callus under
selective conditions.
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Note: Patent information on this page was last updated on 7 February 2006.
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