Pineapple - Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5952543
- Earliest priority - 25 February 1997
- Filed - 24 February 1998
- Granted - 14 September 1999
- Expected expiry - 23 February 2018
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Title - Genetically transformed pineapple plants and methods
for their production
| Claim 1
A method for modifying the genotype of a pineapple cell, said method
comprising:
A) contacting said pineapple cell with Agrobacterium comprising a
T-DNA containing a DNA segment, such that said DNA segment is integrated into
the genome of said pineapple cell; and
B) selecting a pineapple cell
comprising said integrated DNA segment wherein said pineapple cell is an
embryogenic cell or an embryogenic callus cell.
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| Claim 11
A method for modifying the genotype of a pineapple cell, said method
comprising:
A) culturing pineapple tissue to produce pineapple embryogenic cells;
B) contacting said pineapple embryogenic cells with Agrobacterium
comprising a T-DNA containing a DNA segment, such that said DNA segment is
integrated into the genome of said pineapple cells; and
C) selecting a
pineapple cell comprising said integrated DNA segment.
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DNA Plant Technology Corp.
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AU
740294 B2
- Earliest priority - 25 February 1997
- Filed - 25 February 1998
- Granted - 1 November 2001
- Expected expiry - 24 February 2018
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Title - Genetically transformed pineapple plants and methods
for their production
| Claim 1
A method for modifying the genotype of a pineapple cell, said method
comprising:
A) contacting said pineapple cell with Agrobacterium comprising a
T-DNA containing a DNA segment, such that said DNA segment is integrated into
the genome of said pineapple cell; and
B) selecting a pineapple cell
comprising said integrated DNA segment wherein said pineapple cell is an
embryogenic cell or an embryogenic callus cell.
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| Claim 11
A method for modifying the genotype of a pineapple cell, said method
comprising:
A) culturing pineapple tissue to produce pineapple embryogenic cells;
B) contacting said pineapple embryogenic cells with Agrobacterium
comprising a T-DNA containing a DNA segment, such that said DNA segment is
integrated into the genome of said pineapple cells; and
C) selecting a
pineapple cell comprising said integrated DNA segment.
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| Claim 39
A method for modifying the genotype of a pineapple cell, said method being
substantially as hereinbefore described with reference to any one of the
examples.
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| Claim 41
A pineapple plant cell comprising an integrated Agrobacterium T-DNA
sequence comprising a heterologous gene, substantially as hereinbefore described
with reference to any one of the examples.
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| Claim 51
A pineapple plant comprising an integrated Agrobacterium T-DNA
sequence comprising a heterologous gene, substantially as hereinbefore described
with reference to any one of the examples.
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* Claims 1 and 11 of the Australian granted patent are the same as those of
the United States patent. The three additional independent claims in this patent
(claims 39, 41, 51) recite methods and transgenic pineapple plants stated in the
examples of the specification.
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WO
2001/33943 A1
- Earliest priority - 5 November 1999
- Filed - 3 November 2000
- OPI - 17 May 2001
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Title - A method of plant transformation
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Claim 1 - See below *
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Claim 21 - See below *
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Claim 41 - See below *
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| Claim 60
A method of transforming cells of a pineapple plant or a related plant with
genetic material, said method comprising: A) obtaining explant from said
pineapple plant or a related species;
B) co-cultivating same with
Agrobacterium species having T-DNA or T-DNA region comprising genetic
material to be transformed into said pineapple plant cells for a time and under
conditions sufficient for transfer of the genetic material to occur;
C)
selecting for transformed pineapple or related cells and permitting the cells to
form organogenic callus.
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| Claim 77
A method of genetically modifying a pineapple or related plant, said method
comprising: A) obtaining an explant from a pineapple or related plant to be
genetically modified;
B) co-cultivating the explant with
Agrobacterium species having a T-DNA comprising genetic material to be
transferred into the pineapple or related cells for a time and under conditions
sufficient for the genetic material to transfer to said cells;
C) selecting
for transformed pineapple or related cells and permitting the cells to form
organogenic callus; and
D) regenerating a pineapple or related plant from
said selected transformed cells.
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Method for transforming pineapple cells by co-cultivating an explant with
Agrobacterium having T-DNA with genetic material to be transformed into
the plant cells.
The selected transformed pineapple cells form an organogenic callus. A
genetically modified pineapple plant is regenerated from the organogenic callus.
* The present application also contains claims (1, 21, 41) directed to
methods for transforming monocot plants in general, which are discussed under
the section
General
transformation methods.
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Department of Primary Industries of Queensland
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AU
779510 B
- Earliest priority - 5 November 1999
- Filed - 3 November 2000
- Granted - 27 January 2005
- Expected expiry - 2 November 2020
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Title - A method of plant transformation
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Claim 1
A method of transforming cells of a pineapple plant with genetic material,
said method comprising:-
(a) obtaining an explant from said plant;
(b) co-cultivating the explant with Agrobacterium species having a
T-DNA or T-DNA region comprising the genetic material to be transformed into the
plant cells for a time and under conditions sufficient for the genetic material
to transfer into the plant cells without said Agrobacterium overgrowing the
plant cells; and
(c) selecting for the tranformed plant cells and permitting the cells to form
organogenic callus.
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Claim 19
A method for producing a genetically modified pineapple plant, said method
comprising:-
(a) obtaining explant from a plant to be genetically modified;
(b) co-cultivating the explant with Agrobacterium species having a
T-DNA or T-DNA region comprising genetic material to be transformed into said
plant cells for a time and under conditions sufficient for the genetic material
to transfer to plant cells without said Agrobacterium overgrowing the plant
cells;
(c) selecting transformed plant cells and permitting the cells to form
organogenic callus; and
(d) then regenerating a plant from selected transformed plant cells.
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Claim 37
A method for producing a genetically modified pineapple plant, said method
comprising:
(a) obtaining an explant from said plant to be genetically modified;
(b) co-cultivating the explant with Agrobacterium species having a
T-DNA or T-DNA region comprising the genetic material to be transformed into the
plant cells for a time and under conditions sufficient for the genetic material
to transfer into the plant cells without the Agrobacterium overgrowing the plant
cells;
(c) selecting for the transformed plant cells and permitting the cells to
form organogenic callus; and
(d) regenerating a plant from said transformed organogenic callus.
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Granted independent claims in this patent recite the same method of pineapple
transformation as claims 60 and 77 in WO 2001/33943, except
that the plant is limited to pineapple only and not those of "related plants".
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Note: Patent information on this page was last updated on 7 February 2006.
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