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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6329571
- Earliest priority - 22 October 1996
- Filed - 22 October 1997
- Granted - 11 December 2001
- Expected expiry - 21 October 2017
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Title - Method for Transforming Indica Rice
| Claim 1
A method for transforming rice comprising transforming immature embryo cells
of Indica rice by Agrobacterium method and selecting transformed cells,
characterized in that a medium containing
- 2000 to 4000 mg/l of KNO3,
- 60 to 200 mg/l of MgSO4,
- 200 to 600 mg/l of KH2PO4,
- 100 to 450 mg/l of CaCl2,
- 200 to 600 mg/l of (NH4)2SO4,
- 1 to 7 mg/l of H3BO3,
- 2 to 20 mg/l of MnSO4,
- 20 to 50 mg/l of EDTA or a salt thereof,
- 3 to 8 mg/l of Fe,
- 50 to 200 mg/l of myoinositol,
- 0.5 to 10 mg/l of 2,4-dichlorophenoxyacetic acid,
- 0.01 to 5 mg/l of a cytokinin,
- 5000 to 80,000 mg/l of a sugar, and
- a gelling agent,which medium has a pH of 4.5 to 6.5,
is used as a medium for selecting said transformed cells.
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A method for transformation of Indica rice using immature embryo cells as
target tissue for Agrobacterium. Ranges for the media components are
disclosed in the independent claim.
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Japan Tobacco
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AU
736027 B2
- Earliest priority - 22 October 1996
- Filed - 22 October 1997
- Granted - 26 July 2001
- Expected expiry - 21 October 2017
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Title - Method for Transforming Indica Rice
| Claim 1
A method for transforming rice comprising transforming immature embryo cells
of Indica rice by Agrobacterium method and selecting transformed cells,
characterized in that a medium containing:
- 2000 to 4000 mg/l of KNO3,
- 60 to 200 mg/l of MgSO4,
- 200 to 600 mg/l of KH2PO4,
- 100 to 450 mg/l of CaCl2,
- 200 to 600 mg/l of (NH4)2SO4,
- 1 to 7 mg/l of H3BO3,
- 2 to 20 mg/l of MnSO4,
- 20 to 50 mg/l of EDTA or a salt thereof,
- 3 to 8 mg/l of Fe,
- 50 to 200 mg/l of myoinositol,
- 0.5 to 10 mg/l of 2,4-dichlorophenoxyacetic acid,
- 0.01 to 5 mg/1 of a cytokinin,
- 5000 to 80,000 mg/l of a sugar, and
- a gelling agent, which medium has a pH of 4.5 to 6.5,
is used as a medium for selecting said transformed cells.
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Transformation of immature embryo cells of Indica rice by
Agrobacterium. The composition of the medium used to select the
transformed cells is disclosed.
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EP 897013
A1
- Earliest priority - 22 October 1996
- Filed - 27 October 1997
- Application deemed withdrawn - 18 December 2003
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Title - Method for Transforming Indica Rice
| Claim 1
A method for transforming rice comprising transforming immature embryo cells
of Indica rice by Agrobacterium method and selecting transformed cells,
characterized in that a medium containing:
- 2000 to 4000 mg/l of KNO3,
- 60 to 200 mg/l of MgSO4,
- 200 to 600 mg/l of KH2PO4,
- 100 to 450 mg/l of CaCl2,
- 200 to 600 mg/l of (NH4)2SO4,
- 1 to 7 mg/l of H3BO3,
- 2 to 20 mg/l of MnSO4,
- 20 to 50 mg/l of EDTA or a salt thereof,
- 3 to 8 mg/l of Fe,
- 50 to 200 mg/l of myoinositol,
- 0.5 to 10 mg/l of 2,4-dichlorophenoxyacetic acid,
- 0.01 to 5 mg/1 of a cytokinin,
- 5000 to 80,000 mg/l of a sugar, and
- a gelling agent, which medium has a pH of 4.5 to 6.5,
is used as a medium for selecting said transformed cells.
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Remarks
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- National phase entry of
WO
1998/17813 in Canada (CA 2240454) has lapsed according to CIPO.
- National phase entry of
WO
1998/17813 in China (CN 1206435) is deemed withdrawn according to CNPO.
- National phase entry of
WO
1998/17813 in Japan (JP H10-117776) has been rejected by the JPO.
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