Rice - Specific Patent Information - part 2
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6215051
- Earliest priority - 4 November 1992
- Filed - 4 May 1998
- Granted - 10 April 2001
- Expected expiry - 3 May 2018
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Title - Aarobacterium-mediated method for
transforming rice
(note that Agrobacterium has the incorrect
spelling, as shown above, on the patent)
| Claim 1
A method for the production of a transgenic plant of rice crop comprising the
steps:
A) infecting an immature embryo of rice crop with the genus
Agrobacterium for transformation;
B) co-culturing the infected
embryo with a dicot suspension culture during the step of transformation;
C) allowing the transformed embryo in step (B) to grow into a callus in a
selective medium comprising a sufficient amount of a plant growth hormone for
the growth of rice crop; and
D) allowing the cultured callus to regenerate
root and shoot in a regeneration medium comprising a pre-determined amount of
nutrients for the growth of rice crop.
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| Claim 8
A method for the production of a transgenic rice plant comprising the steps
of:
A) transforming an immature rice embryo with a gene encoding a desired gene
product by culturing the embryo in a dicot suspension culture with bacteria from
the genus Agrobacterium, said bacteria comprising said gene;
B)
growing the transformed embryo from step (A) into a callus in a selective medium
comprising a rice plant growth hormone; and
C) regenerating root and shoot
from said cultured callus in a regeneration medium comprising nutrients for the
growth of rice crop.
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Method for the production of transgenic rice plants by co-culturing an
immature rice embryo and Agrobacterium with a dicot suspension
culture. The transformed embryo grows into a callus that in turn
regenerates roots and shoots.
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National Science Council of R.O.C.
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Remarks
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Related Japanese applications and United States patents are not directed to
transformation of rice with Agrobacterium. They refer to a gene
expression system with a promoter region from the alpha amylase genes.
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WO
2001/12828 A1
- Earliest priority - 18 August 1999
- Filed - 17 August 2000
- OPI - 22 February 2001
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Title - Methods and Apparatus for transformation of
Monocotyledenous plants using Agrobacterium in combination with vacuum
filtration
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Claim 1 - See below *
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| Claim 20
An in planta method of transforming a rice plant comprising: A)
contacting at least one panicle of the rice plant with a solution or suspension
comprising at least one Agrobacterium clone; and
B) subjecting the
rice plant to a vacuum effective to cause entry of the Agrobacterium
clone into at least one flower of the panicle.
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| Claim 38
An in planta method of producing a transgenic rice plant,
comprising: A) contacting at least one panicle of a first rice plant with a
solution comprising at least one Agrobacterium clone wherein the
Agrobacterium clone comprises at least one heterologous gene;
B)
subjecting the first rice plant to a vacuum effective to cause entry of the
Agrobacterium clone into at least one flower of the panicle;
C)
cultivating the first rice plant to maturity; and
D) collecting seeds of
the first rice plant expressing the heterologous gene.
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Claim 40 - See below *
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An in planta method for transforming rice panicles by contacting the
panicle with Agrobacterium in a suspension containing a heterologous
gene and subjecting the plant to vacuum so Agrobacterium enters the
plant part. After the transformation the plant is cultivated into maturity and
seeds express the heterologous gene.
* Independent claims 1 and 40 recite use of vacuum infiltration to transform
monocotyledonous plants, and are introduced in the
General
Monocot Transformation Methods section.
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Paradigm Genetics
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Remarks
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National phase entry of WO 01/12828 in Australia (AU 67807/00 A) has lapsed.
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WO
02/057407 A2
- Earliest priority - 17 January 2001
- Filed - 14 January 2002
- OPI - 25 July 2002
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Title - Novel Method for Transgenic Plants by Transformation
and Regeneration of Indica Rice Plant Shoot Tips
| Claim 1
A novel method of transforming excised shoot tip tissue of the Indica rice
cultivars by using the Agrobacterium method.
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A method of transformation of Indica rice using excised shoot tip tissue as a
target for Agrobacterium.
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Avestha Gengraine Technologies Pty. Ltd.
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Remarks
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- related application in India IN 2001CH00047
- National phase entry of WO 02/57407 in Europe (EP 1444339) is still pending.
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Note: Patent information on this page was last updated on 8 February 2006.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
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