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AU
738153 C
- Earliest priority - 21 June 1996
- Filed - 20 June 1997
- Granted - 13 September 2001
- Amended - 1 July 2004
- Expected expiry - 19 June 2017
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Title - Methods for the production of stably-transformed,
fertile wheat employing agrobacterium-mediated transformation and
compositions derived therefrom
| Claim 1
A fertile, transgenic wheat plant, the genome of which has been altered
through the genomic introduction of a pre-selected genetic component, said
component comprising an exogenous gene positioned under the control of one or
more pre-selected genetic control elements, the plant prepared by a process
comprising:
A) preparing a DNA composition in vitro, which composition includes
the genetic component one desires to introduce into the genome of a wheat plant;
B) introducing said DNA composition into recipient wheat cells by
Agrobacterium transformation; C) regenerating wheat plants from
said cells which have received said genetic component; and D) identifying a
fertile, transgenic wheat plant whose genome has been altered through the stable
introduction of said genetic component, and wherein said recipient cells
comprise an immature embryo, a callus tissue, or suspension cells.
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| Claim 7
A fertile, transgenic wheat plant, the genetic complement of which has been
altered through the addition of a DNA composition comprising a pre-selected
functional genetic element that includes a transgene selected from the group
consisting of an nptll gene, a bla gene, a nptI gene,
a dhfr gene, a aphlV gene, a aacC3 gene, a
aacC4 gene and a GUS gene, wherein said functional genetic element
confers on said wheat plant a phenotypic trait that is not found in the
parentage of said plant, wherein said DNA composition was added using
Agrobacterium transformation of recipient cells comprising an immature
embryo, a callus tissue, or suspension cells.
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| Claim 10
A fertile, transgenic wheat plant, the genome of which has been altered
through the genomic introduction of a pre-selected genetic component, said
component comprising an exogenous gene positioned under the control of one or
more pre-selected genetic control elements, substantially as hereinbefore
described with reference to any one of the examples.
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Claim 11
A fertile, transgenic wheat plant, the genetic complement of which has been
altered through the addition of a DNA composition comprising a pre-selected
functional genetic element, substantially as hereinbefore described with
reference to any one of the examples.
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| Claim 14
A method for producing a fertile transgenic wheat plant, comprising the steps
of:
A) establishing a regenerable culture from a wheat plant to be transformed,
wherein said culture comprises an immature embryo, a callus tissue, or
suspension cells; B) introducing a DNA composition comprising a genetic
component one desires to introduce into the genome of said wheat plant, by
Agrobacterium transformation; C) identifying or selecting a
transformed cell line; and D) regenerating a fertile transgenic wheat plant
therefrom, wherein said DNA is transmitted through a complete sexual cycle of
said transgenic plant to its progeny, wherein said progeny comprises a
selectable or screenable marker gene, and wherein said marker gene is
chromosomally integrated.
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| Claim 18
A method for producing a fertile transgenic wheat plant, substantially as
hereinbefore described with reference to any one of the examples.
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| Claim 19
A method for producing a transgenic wheat plant, comprising the steps of:
A) establishing a culture from a wheat plant to be transformed, wherein said
culture comprises an immature embryo, a callus tissue, or suspension cells;
B) transforming said culture with an Agrobacterium comprising a
DNA composition comprising a genetic component one desires to introduce into the
genome of said wheat plant; C) identifying or selecting a transformed cell
line; and D) regenerating a transgenic wheat plant therefrom.
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| Claim 21
A method for producing a transgenic wheat plant, substantially as
hereinbefore described with reference to any one of the examples.
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Process for the production of a fertile, transgenic wheat plant by
transforming an immature embryo, a callus tissue or suspension cells of wheat
with Agrobacterium having an exogenous gene. The transgene can be
selected from: an nptII gene, bla gene, nptI gene, dhfr gene, aphIV gene, aacC3,
aacC4 and a GUS gene. The fertile transgenic wheat plant produces progeny
comprising a selectable or screenable marker integrated into the chromosomes.
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Monsanto
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EP
856060 A1
- Earliest priority - 21 June 1996
- Filed - 20 June 1997
- Application pending
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Title - Methods for the Production of Stably-transformed,
Fertile Wheat employing Agrobacterium-mediated transformation and
compositions derived therefrom
| Claim 1
A fertile, transgenic wheat plant, the genome of which has been altered
through the genomic introduction of a pre-selected genetic component, said
component comprising an exogenous gene positioned under the control of one or
more pre-selected genetic control elements, the plant prepared by a process
comprising:
A) preparing a DNA composition in vitro, which composition includes
the genetic component one desires to introduce into the genome of a wheat plant;
B) introducing said DNA composition into recipient wheat cells by
Agrobacterium transformation; C) regenerating wheat plants from
said cells which have received said genetic component; and D) identifying a
fertile, transgenic wheat plant whose genome has been altered through the stable
introduction of said genetic component.
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| Claim 8
A fertile, transgenic wheat plant, the genetic complement of which has been
altered through the addition of a DNA composition comprising a pre-selected
functional genetic element that includes a transgene selected from the group
consisting of an nptll gene, a bla gene, a nptI gene, a dhfr gene, a aphlV gene,
a aacC3 gene, a aacC4 gene and a GUS gene, wherein said functional genetic
element confers on said wheat plant a phenotypic trait that is not found in the
parentage of said plant.
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| Claim 14
A method for producing a fertile transgenic wheat plant, comprising the steps
of:
A) establishing a regenerable culture from a wheat plant to be transformed;
B) introducing a DNA composition comprising a genetic component one
desires to introduce into the genome of said wheat plant, by
Agrobacterium transformation; C) identifying or selecting a
transformed cell line; and D) regenerating a fertile transgenic wheat plant
therefrom, wherein said DNA is transmitted through a complete sexual cycle of
said transgenic plant to its progeny, wherein said progeny comprises a
selectable or screenable marker gene, and wherein said marker gene is
chromosomally integrated.
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| Claim 18
A method for producing a transgenic wheat plant, comprising the steps of:
A) establishing a culture from a wheat plant to be transformed; B)
transforming said culture with an Agrobacterium comprising a DNA
composition comprising a genetic component one desires to introduce into the
genome of said wheat plant; C) identifying or selecting a transformed cell
line; and D) regenerating a transgenic wheat plant therefrom.
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National phase entry of WO 97/48814, this application is still pending.
Insertion of a foreign gene into a wheat plant via Agrobacterium .
The foreign gene is selected from a group of genes conferring resistance to
different antibiotics, i.e. kanamycin, hygromycin, and also the GUS gene.
Regenerating and obtaining fertile transformed wheat plants is also covered by
the claims.
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