Patent assigned to Rhône-Poulenc Agro

The invention disclosed in the following patent is directed to the use of an inflorescence of a monocot as a target tissue to be transformed with Agrobacterium. In the method, a dissected inflorescence or a callus derived from the dissected inflorescence is co-cultivated with Agrobacterium.

Specific Patent Information

Patent Number

Title, Independent Claims and Summary of Claims

Assignee

US 6037522

Earliest priority - 23 June 1998
Filed - 23 June 1998
Granted - 14 March 2000
Expected expiry - 22 June 2018

Title- Agrobacterium-mediated transformation of monocots

Claim 1

A method of transforming a monocot comprising co-cultivating a monocot inflorescence with Agrobacterium containing a plasmid comprising a heterologous nucleic acid.

Claim 5

A method of transforming a monocot comprising:

A) dissecting an inflorescence from a monocot;
B) initiating a callus from the inflorescence to generate an inflorescence-derived callus; and
C) co-cultivating the inflorescence-derived callus with Agrobacterium containing a plasmid comprising a heterologous nucleic acid.

Claim 13

A method of making a transgenic monocot comprising:

A) dissecting an inflorescence from a monocot;
B) initiating a callus from the inflorescence to generate an inflorescence-derived callus;
C) co-cultivating the inflorescence-derived callus with Agrobacterium containing a plasmid comprising a heterologous nucleic acid; and
D) regenerating a transgenic monocot from the callus.

Claim 16

A method of making a transgenic monocot comprising:

A) dissecting an inflorescence from a monocot;
B) co-cultivating the inflorescence with Agrobacterium containing a plasmid comprising a heterologous nucleic acid;
C) initiating a callus from the inflorescence; and
D) regenerating a transgenic monocot from the callus.

The United States patent US 6037522 claims

a method of transforming an inflorescence of a monocot with Agrobacterium having heterologous DNA where:

the inflorescence to be transformed is first dissected and then co-cultivated with Agrobacterium; or
the inflorescence to be transformed is first cultured in a medium to induce callus formation and then the callus is transformed with Agrobacterium.

Dependent claims also recite transgenic monocots, seeds or progeny of transgenic monocots produced by the methods.

The patent describes that "heterologous nucleic acid" is nucleic acid which is not normally found in Agrobacterium T-DNA or the monocot that is to be transformed and includes all synthetically engineered and biologically derived genes which may be introduced into a plant by genetic engineering, including, but not limited, to nonplant genes, modified genes, synthetic genes, portion of genes, and genes from monocots and other plant species.

Rhône-Poulenc Agro

(now Aventis CropScience,
now owned by Bayer)

Remarks

The related Australian application AU 46163/99 A1 was abandoned on March 15, 2001.

Note: Patent information on this page was last updated on 2 February 2006.

The information contained in this page was believed to be correct at the time it was collated. New patents and patent applications, altered status of patents, and case law may have resulted in changes in the landscape. CAMBIA makes no warranty that it is correct or up to date at this time and accepts no liability for any use that might be made of it. Corrections or updates to the information are welcome. Please send an email to info@bios.net.

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