Acacia
Summary
The genus Acacia is composed of as many as 8000 species native to
the tropics and subtropics. The commercially valuable species Acacia
mangium is variously known as "Mangium", "Black Wattle" and "Hickory
Wattle", and is traded as brown salwood. Its distribution covers the northern
part of Queensland in Australia, Papua New Guinea and the Molucca Islands of
Indonesia. Mangium is a fast-growing tree, relatively short-lived (30-50 years)
and adapted to a wide range of acidic soils in most tropical lowlands. It is a
pioneer species that colonize disturbed sites. Major plantations of A.
mangium are grown in Southeast Asia for paper pulp. Wood from this species
is used in furniture, handles of sporting goods, door frames and light
construction. Plantations of A. mangium are also used for erosion
control, shade and shelter. This species is playing an important role in efforts
to sustain commercial supply of tree products while reducing pressure on natural
forest ecosystems.
The Institute of Molecular Agrobiology, located at the
National University of Singapore, has filed applications related to the genetic
transformation of A. mangium with Agrobacterium and to the
regeneration of the species. The PCT applications disclose methods for
transforming A. mangium with a gene of interest using A.
tumefaciens. One of the recited methods uses stems as initial tissue to be
transformed with the bacterium.
For patent documents in the United States, Australia and New Zealand, the
ownership has changed to Temasek Life Sciences Laboratory Ltd.
Specific Patent Information
|
Patent Number
|
Title, Independent Claims and Summary of Claims
|
Assignee
|
|
US
6846971
- Earliest priority - 19 January 2000
- Filed - 2 January 2002
- Granted - 25 January 2005
- Expected expiry - 18 January 2000
|
Title - Regeneration and genetic transformation of
Acacia mangium
Claim 1
A method of transforming Acacia mangium with a gene of interest
comprising the steps of:
a) activating Agrobacterium tumefaciens comprising said gene of
interest by culturing said Agrobacterium in induction medium comprising
acetosyringone;
b) preculturing an explant of Acacia mangium
selected from the group comprising a stem, a leaflet, a petiole and a bud in
medium comprising supplemented basic MS medium, wherein said explant is soaked
in 0.5 M mannitol prior to the co-cultivation;
c) co-cultivating said
activated Agrobacterium tumefaciens and said precultured explant in
medium comprising supplemented basic MS medium to produce infected
explants;
d) culturing said infected explants in medium comprising
supplemented basic MS to induce callus and adventitious buds; and
e)
culturing said callus or adventitious buds on a selective medium comprising
supplemented basic MS medium; wherein in said supplemented basic MS medium
comprises a) thidiazuron, b) indole-3-acetic acid, c) casein enzymatic
hydrolysate, d) L-ascorbic acid, e) L-glutamine, f) L-asparagine, g) L-proline,
h) sucrose and l) phytagel or agar.
|
Claim 7
A method of preparing transgenic Acacia mangium cells comprising the
steps of
a) preculturing stem pieces of Acacia mangium in culture
medium comprising supplemented basic MS medium, wherein said stem pieces are
soaked in 0.5M mannitol prior to co-cultivating with Agrobacterium
tumefaciens wherein said supplemented basic MS medium comprises a)
thidiazuron, b) indole-3-acetic acid, c) casein enzymatic hydrolysate, d)
L-ascorbic acid, e) L-glutamine, f) L-asparagine, g) L-proline, h) sucrose and
i) phytagel or agar; and
b) co-cultivating said stem pieces of step (a)
with Agrobacterium tumefaciens in culture medium AM-265, wherein said
Agrobacterium was activated by culturing the Agrobacterium in
induction medium comprising acetosyringone prior to said co-cultivation.
|
Granted US 6846971 recites a method of
Agrobacterium-mediated transformation of Acacia mangium.
|
Temasek Life Sciences Laboratory Ltd
|
|
AU
784180 B2
- Earliest priority - 19 January 2000
- Filed - 19 January 2000
- Granted - 12 February 2006
- Expected expiry - 18 January 2020
|
Title - Regeneration and genetic transformation of
Acacia mangium
|
Caim 1
A method for regenerating Acacia mangium
comprising:
a) inducing callus formation from an explant;
b) culturing
said callus to produce adventitious buds;
c) culturing said adventitious
buds to elongate and produce pinnate leaves; and
d) culturing elongated
buds of step (c) such that they produce roots and become plantlets.
|
|
Claim 11
A method for regenerating Acacia mangium
comprising:
a) culturing auxiliary buds from an Acacia mangium
tree to produce adventitious buds comprising hyllodes;
b) subculturing said
adventitious buds comprising phyllodes to produce advantitious shoots;
c)
culturing said adventitious shoots.
|
|
Claim 14
A method of tranforming Acacia
mangium with a gene of interest comprising the steps of:
a)
activating Agrobacterium tumefaciens comprising said
gene of interest to form activated Agrobacterium tumefaciens;
b)
preculturing an explant of Acacia mangium to yield a precultured
explant;
c) co-cultivating said activated Agrobacterium
tumefaciens and said precultured explant to produce infected
explants;
d) culturing said infected explants to induce callus and
adventitious buds; and
e) culturing said callus or adventitious buds on a
selective medium.
|
|
Claim 25
A method for promoting root formation from
transformed adventitious buds comprising
- culturing transformed adventitious buds on a medium comprising 1/2 MS basic
medium supplemented with a) alpha-naphthaleneacetic acid, b) kinetin, c) casein
enzymatic hydrolysate, d) L-ascorbic acid, e) L-glutamine, f) L-asparagine, g)
L-proline, h) sucrose and i) phytagel.
|
|
Claim 28
A method of preparing transgenic Acacia
mangium cells comprising the steps of
a) preculturing stem pieces of
Acacia mangium in a culture medium; and
b) cocultivating said
stem pieces of step (a) with activated Agrobacterium tumefaciens.
|
|
Claim 41
A transgenic Acacia mangium cell.
|
|
Claim 42
A transgenic Acacia mangium plant.
|
The applicant of granted AU 784180 is stated in the patent
document as Temasek Life Sciences Laboratory.
|
|
US
2005/155116 A1
- Earliest priority - 19 January 2000
- Filed - 9 December 2004
- Application pending
|
Title - Regeneration and genetic transformation of acacia
mangium
|
Claim 1
A method for regenerating Acacia mangium
comprising:
a) inducing callus formation from an explant;
b) culturing
the callus on a medium comprising thidiazuron to produce adventitious
buds;
c) culturing the adventitious buds to elongate the buds and produce
pinnate leaves; and
d) culturing elongated buds of step (c) to produce
plantlets with roots.
|
|
Claim 11
A method for regenerating Acacia mangium
comprising:
a) culturing auxiliary buds from an Acacia mangium
tree to produce adventitious buds comprising phyllodes;
b) subculturing the
adventitious buds comprising phyllodes to produce adventitious shoots;
c)
culturing the adventitious shoots.
|
|
Claim 14
A method of making transgenic Acacia
mangium plants comprising;
a) preparing transgenic Acacia
mangium cells by preculturing stem pieces in culture medium comprising
thidiazuron;
b) co-cultivating the stem pieces of step (a) with
Agrobacterium in medium comprising thidiazuron to form adventitious
buds;
c) culturing the adventitious buds in a selective medium comprising
an antibiotic and gibberellic acid; and
d) rooting the plantlets which
develop from the adventitious buds of step c).
|
This application is a divisional of now granted US 6846971
(see above).
|
|
WO
2001/53452 A2
- Earliest priority - 19 January 2000
- Filed - 19 January 2000
- OPI - 7 November 2002
|
Title - Regeneration and genetic transformation of acacia
mangium
|
Claim 1
A method for regenerating Acacia mangium comprising:
A) inducing callus formation from an explant;
B) culturing said callus
to produce adventitious buds;
C) culturing said adventitious buds to
elongate and produce pinnate leaves; and
D) culturing elongated buds of
step (c) such that they produce roots and become plantlets.
|
|
Claim 11
A method for regenerating Acacia mangium comprising:
A) culturing auxiliary buds from an Acacia mangium tree to produce
adventitious buds comprising phyllodes;
B) subculturing said adventitious
buds comprising phyllodes to produce adventitious shoots; and
C) culturing
said adventitious shoots.
|
|
Claim 14*
A method of transforming Acacia mangium with a gene of interest
comprising the steps of:
A) activating Agrobacterium tumefaciens comprising said gene of
interest to form activated Agrobacterium tumefaciens;
B)
preculturing an explant of Acacia mangium to yield a precultured
explant;
C) co-cultivating said activated Agrobacterium
tumefaciens and said precultured explant to produce infected
explants;
D) culturing said infected explants to induce callus and
adventitious buds; and
E) culturing said callus or adventitious buds on a
selective medium.
|
|
Claim 25
A method for promoting root formation from transformed adventitious buds
comprising culturing transformed adventitious buds on a medium comprising 1/2 MS
basic medium supplemented with:
A) cx-naphthaleneacetic acid,
B) kinetin,
C) casein enzymatic
hydrolysate,
D) L-ascorbic acid,
E) L-glutamine,
F)
L-asparagine,
G) L-proline,
H) sucrose and
I) phytagel.
|
| Claim 28*
A method of preparing transgenic Acacia mangium cells comprising the
steps of:
a) preculturing stem pieces of Acacia mangium in a culture medium;
and
b) co-cultivating said stem pieces of step (a) with activated
Agrobacterium tumefaciens.
|
| Claim 41
A transgenic Acacia mangium cell.
|
| Claim 42
A transgenic Acacia mangium plant.
|
Methods for transforming explants of A. mangium with a gene of
interest by co-cultivating A. tumefaciens having the gene of interest
with pre-cultured explants of the tree species. In one of the methods the tissue
selected for transformation is stems. Transgenic cells and plants of A.
mangium are also part of the recited invention.
Additional claims recite methods for the regeneration of non-transformed
A. mangium from different tissues.
|
Institute of Molecular Agrobiology
|
| Remarks |
- National phase entries of WO 2001/53452 in Canada (CA 2362767) and China (CN
1378599) are pending.
- National phase entry of WO 2001/53452 in Europe (EP 1272608) is deemed to be
withdrawn on 9 March 2005.
- National phase entry of WO 2001/53452 in New Zealand (NZ 514910) has been
granted on 27 February 2004.
- Other national phase entry of WO 2001/53452 include: Norway (NO 20014331;
application rejected according to INPADOC), Brazil (BR 200009112).
|
|
Note: Patent information on this page was last updated on 10 March 2006.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
status of patents, and case law may have resulted in changes in the
landscape. CAMBIA makes no warranty that it is correct or up to date at
this time and accepts no liability for any use that might be made of it.
Corrections or updates to the information are welcome. Please send an email to
info@bios.net.
There are no comments.