Cotton - Patents granted to Agracetus

A method of introducing genes into cotton plants and plant lines comprising the steps of:

A) exposing hypocotyl tissue of immature cotton plants to a culture of transformation competent non-oncogenic Agrobacterium tumefaciens harboring a Ti plasmid having a T-DNA region including both a foreign chimeric gene and a selection agent resistance gene, both genes including appropriate regulatory sequences so as to be expressed in the cells of cotton plants;
B) culturing the exposed tissue in the presence of a selection agent for which the resistance gene encodes for resistance so as to select for plant cells transformed with the T-DNA region;
C) inducing somatic embryo formation in the exposed tissue in culture; and
D) regenerating the somatic embryos into whole cotton plants.

A method for introducing genes into cotton plants and plant lines, comprising the following steps in sequence:

A) surface sterilizing cotton seeds;
B) allowing said cotton seeds to germinate thus forming immature cotton plants, said immature cotton plants including hypocotyl tissue;
C) exposing said hypocotyl tissue to a culture of transformation competent non-oncogenic Agrobacterium tumefaciens harboring a Ti plasmid having a T-DNA region including both a foreign chimeric gene and a selection agent resistance gene;
D) culturing said hypocotyl tissue on a medium containing at least one antibiotic toxic to said Agrobacterium tumefaciens but not toxic to cotton cells;
E) culturing said tissue of step (D) in the presence of a selection agent for which the resistance gene encodes for resistance so as to select for plant cells transformed with the T-DNA region;
F) inducing somatic embryo formation in the exposed tissue in culture; and
G) regenerating the somatic embryos into whole cotton plants.

Cotton seed capable of germination into a cotton plant comprising in its genome a chimeric recombinant gene construction including:

(i) a foreign gene, and
(ii) promoter and control sequences operable in cotton cells,

• the chimeric gene construction being effective in the cells of the cotton plant to express a cellular product coded by the foreign gene;
• the cellular product imbuing the plant with a detectable trait;
• the cellular product selected from the group consisting of a foreign protein and a negative strand RNA.

A cotton plant comprising in the genome of at least some of its cells a foreign gene construction including promoter and control sequences effective in cotton cells,

• said gene construction further including a heterologous coding sequence;
• the foreign gene construction effective to cause expression of a detectable cellular product coded by the heterologous coding sequence in the plant cells;
• the cellular product selected from the group consisting of a foreign protein and a negative strand RNA.

A cotton plant comprising in its genome at least two foreign gene constructions each including promoter and control sequences effective in cotton cells,

• both gene constructions further including heterologous coding sequences;
• both foreign gene constructions effective to cause the expression of a detectable cellular product coded by the heterologous coding sequence in the plant cells;
• the cellular product of one of the foreign gene constructions selected from the group consisting of a foreign protein and a negative strand RNA;
• the other foreign gene construction being a selectable marker gene which imbues the cotton cells with the trait of resistance to a selection agent.

A cotton plant comprising in its genome at least two foreign gene constructions each including promoter and control sequences effective in cotton cells,

• both gene constructions further including heterologous coding sequences;
• both foreign gene constructions effective to cause the expression of a detectable cellular product coded by the heterologous coding sequence in the plant cells;
• the cellular product of one of the foreign gene constructions selected from the group consisting of a foreign protein and a negative strand RNA;
• the other foreign gene construction being a selectable marker gene which imbues the cotton cells with the trait of resistance to a selection agent;
• the foreign gene constructions having been transformed into the cotton plant or the progenitors of the cotton plant by Agrobacterium-mediated plant transformation.

A method of introducing genes into cotton plants and plant lines comprising the steps of:

• exposing hypocotyl tissue of immature cotton plants to a culture of transformation competent non-oncogenic Agrobacterium tumefaciens harboring a Ti plasmid having a T-DNA region including both

(i) a foreign chimeric gene, and
(ii) a selection agent resistance gene.

Cotton seeds capable of germination into cotton plants comprising in their genome:

• a chimeric gene construction including:
  (i) a foreign gene, and
  (ii) promoter and control sequences operable in plant cells,
  the chimeric gene construction being effective in the cells of the cotton plant to express a cellular product coded by the foreign gene.