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US
5846797
- Earliest priority - 4 October 1995
- Filed - 4 October 1995
- Granted - 8 December 1998
- Expected expiry - 3 October 2015
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Title - Cotton transformation
| Claim 1
In a method for regenerating transformed cotton plants from explant tissue,
the improvement whereby embryogenic callus is generated from a transformed
cotton tissue explant which is cultivated on cotton callus initiation media
which is not supplied with exogenous plant hormones, wherein said explant tissue
is hypocotyl tissue cut from a seedling which has been grown in the dark.
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| Claim 8
A method for the transformation of cotton plants, said method comprising the
steps of:
A) cutting cotton hypocotyl tissue to form an explant, wherein said hypocotyl
tissue is cut from seedling which has been grown in the dark;
B)
co-cultivating said cotton explant tissue with Agrobacterium
comprising a DNA sequence of interest; and
C) culturing said co-cultivated
explant on cotton callus initiation media comprising a selective agent and no
exogenous plant hormones,
whereby transformed cells are induced to produce
embryogenic callus on said hormone-free selective media.
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Transformation of hypocotyl cotton tissue grown in the dark with
Agrobacterium having a gene of interest. Embryogenic callus induced
from the transformed tissue in a hormone-free medium regenerates into
transformed cotton plants.
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Calgene (now owned by Monsanto)
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EP
910239 B1
- Earliest priority - 4 October 1995
- Filed - 4 October 1996
- Granted - 5 December 2001
- Expected expiry - 3 october 2016
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Title - Transformation of cotton plants
| Claim 1
A method of regenerating cotton plants from explant tissue comprising
generating embryogenic callus from a cotton tissue explant cultivated on cotton
initiation media not supplied with exogenous plant hormones.
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| Claim 12
A method for the transformation of cotton plants, said method comprising:
A) cutting cotton tissue to form an explant;
B) co-cultivating said
cotton explant tissue with Agrobacterium comprising a DNA sequence of
interst; and
C) culturing said co-cultivated explant on cotton initiation
media comprising a selective agent and no exogenous plant hormones,
whereby transformed cells are induced to produce embryogenic callus on said
hormone-free selective media.
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Designated contracting States at the time of grant are: Austria (patent
lapsed as reported by INPADOC), Belgium, Switzerland (patent lapsed as reported
by INPADOC), Germany, Denmark (patent lapsed as reported by INPADOC), Spain,
Finland, France, United Kingdom, Greece, Ireland, Italy, Liechtenstein,
Luxembourg, Monaco, Netherlands (patent lapsed as reported by INPADOC), Portugal
(patent lapsed as reported by INPADOC), Sweden (patent lapsed as reported by
INPADOC)
Unlike the related United States patent, the cotton explant to be transformed
with Agrobacterium is not defined. Any transformed cotton tissue is
induced to produce embryogenic callus on a hormone-free medium.
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AU
727910 B2
- Earliest priority - 4 October 1995
- Filed - 4 October 1996
- Granted - 4 January 2001
- Expected expiry - 3 October 2016
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Title - Transformation of cotton plants
| Claim 1
A method for regenerating transformed cotton plants from explant tissue,
characterized by an improvement whereby embryogenic callus is generated from a
transformed cotton tissue explant which is cultivated on cotton callus
initiation media not supplied which is not supplied with exogenous plant
hormones, wherein said explant tissue is hypocotyl tissue cut from a seedling
which has been grown in the dark.
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| Claim 8
A method for regenerating cotton plants from explant tissue, the method being
substantially as hereinbefore described with reference to any one of the
examples.
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| Claim 9
A method for regenerating cotton plants from explant tissue, the method being
substantially as hereinbefore described with reference to the 'New' Regime in
Figure 1.
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| Claim 11
A method for the transformation of cotton plants, the method comprising the
steps of:
A) cutting cotton hypocotyl tissue to form an explant, wherein said hypocotyl
tissue cut from a seedling which has been grown in the dark;
B)
co-cultivating said cotton explant tissue with Agrobacterium
comprising a DNA sequence of interest; and
C) culturing said co-cultivated
explant on cotton callus initiation media comprising a selective agent and no
exogenous plant hormones,
whereby transformed cells are induced to produce
embryogenic callus on said hormone-free selective media.
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| Claim 14
A method for the transformation of cotton plants, the method being
substantially as hereinbefore described with reference to any one of the
examples.
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The claims of the Australian patent are substantially the same as the claims
of the United States patent. In addition, the Australian patent refers to
methods for regenerating cotton plants.
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WO
1997/12512 A2
- Earliest priority - 4 October 1995
- Filed - 4 October 1996
- OPI - 22 May 1997
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Title - Transformation of cotton plants
Claim 1
In a method for regenerating cotton plants from explant tissue, the
improvement whereby embryogenic callus is generated from a cotton tissue explant
which is not cultivated on cotton initiation media supplied with exogenous plant
hormones.
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Claim 12
A method for the transformation of cotton plants, said method comprising the
steps of
(a) cutting cotton tissue to form an explant,
(b) co-cultivating said
cotton explant tissue with Agrobacterium comprising a DNA sequence of
interest, and
(c) culturing said co-cultivated explant on cotton initiation
media comprising a selective agent and no exogenous plant hormones, whereby
transformed cells are induced to produce embryogenic callus on said hormone-free
selective media.
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