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AU
747514 B2
- Earliest priority - 19 February 1998
- Filed - 18 February 1999
- Granted - 16 May 2002
- Expected expiry - 17 February 2019
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Title - A method for the production of transgenic plants
using apical shoot tips
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Claim 1
A method for producing a transformed plant comprising,
1) isolating apical shoot tips from three day old seedlings;
2) chilling the isolated apical shoot tips for a sufficient period of time to
slow the metabolic activity of cells and to accumulate the cells at a single
stage of cell division or mitosis;
3) dissecting the apical shoot tips to expose meristematic cells;
4) introducing a transforming agent into the dissected apical shoot tips,
wherein said transforming agent is a DNA molecule comprising a gene conferring a
desired phenotypic trait to the plant; and
5) regenerating a transgenic plant from shoots which form on the meristematic
cells.
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Claim 19
A method for producing a transformed cotton plant comprising,
1) isolating apical shoot tips from three-day-old seedlings;
2) chilling
the isolated apical shoot tips for a sufficient period of time to slow the
metabolic activity of cells and to accumulate the cells at a single stage of
cell division or mitosis;
3) dissecting the apical shoot tips to expose
meristematic cells;
4) introducing a transforming agent into the dissected
apical shoot tips, wherein said transforming agent is a DNA molecule comprising
a gene conferring a desired phenotypic trait to the plant; and
5)
regenerating a transgenic plant from shoots which form on the meristematic
cells.
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Claim 22
A transformed cotton plant produced by:
a) isolating aan apical shoot tip from a three day old cotton seedling;
b) chilling the isolated apical shoot tip for a sufficient period of time to
slow the metabolic activity of cells and to accumulate the cells at a single
stage of cell division or mitosis;
c) dissecting the apical shoot tip to
expose meristematic cells;
d) exposing the dissected meristematic cells to
a recombinant Agrobacterium comprising a gene conferring a desired
phenotypic trait to a plant; and
e) regenerating a transgenic cotton plant
from the shoot which form from the meristematic cells.
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Claim 25
A method for producing a transformed cotton plant as is herein described in
the detailed description.
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Cotton Inc.
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EP
1056334 B1
- Earliest priority - 19 February 1998
- Filed - 18 February 1999
- Granted - 8 September 2004
- Expected expiry - 17 February 2019
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Title - A method for the production of
transgenic plants using apical shoot tips
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Claim 1
A method for producing a transformed plant comprising,
a) isolating apical shoot tips from three-day-old-seedlings;
b) chilling
the isolated apical shoot tips for a sufficient period of time to slow the
metabolic activity of cells and to accumulate the cells at a single stage of
cell division or mitosis;
c) dissecting the apical shoot tips to expose
meristematic cells;
d) introducing a transforming agent into the dissected
apical shoot tips, wherein said transforming agent is a DNA molecule comprising
a gene conferring a desired phenotypic trait to the plant; and
e)
regenerating a transgenic plant from shoots which form on the meristematic
cells.
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Claim 7
A method for producing a transformed cotton plant comprising,
a) isolating apical shoot tips from three-day-old seedlings;
b) chilling
the isolated apical shoot tips for a sufficient period of time to slow the
metabolic activity of cells and to accumulate the cells at a single stage of
cell division or mitosis;
c) dissecting the apical shoot tips to expose
meristematic cells;
d) introducing a transforming agent into the dissected
apical shoot tips, wherein said transforming agent is a DNA molecule comprising
a gene conferring a desired phenotypic trait to the plant; and
e)
regenerating a transgenic plant from shoots which form on the meristematic
cells.
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Designated contracting States at the time of grant are: Austria (patent
lapsed as reported by INPADOC), Belgium, Switzerland (patent lapsed as reported
by INPADOC), Cyprus, Germany (patent lapsed as reported by INPADOC), Denmark
(patent lapsed as reported by INPADOC), Spain (patent lapsed as reported by
EPO), Finland (patent lapsed as reported by EPO), France, United Kingdom, Greece
(patent lapsed as reported by EPO), Ireland (patent lapsed as reported by
INPADOC), Italy, Liechtenstein (patent lapsed as reported by EPO), Luxembourg,
Monaco (patent lapsed as reported by EPO), Netherlands (patent lapsed as
reported by INPADOC), Portugal, Sweden (patent lapsed as reported by INPADOC)
Granted EP 1056334 recites a method of
producing a transformed cotton plant, the "transforming agent" of which is not
limited to Agrobacterium.
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WO
1999/041975 A1
- Earliest priority - 19 February 1998
- Filed - 18 February 1999
- OPI - 26 August 1999
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Title - A method for the production of
transgenic plants using apical shoot tips
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Claim 1
A method for producing a transformed plant comprising,
1) isolating apical shoot tips from three day old seedlings;
2) chilling
the isolated apical shoot tips;
3) dissecting the apical shoot tips to
expose meristematic cells;
4) introducing a transforming agent into the
dissected apical shoot tips; and
5) regenerating a plant from shoots which
form on the meristematic cells.
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Claim 23
A method for producing a transformed cotton plant comprising,
1) isolating apical shoot tips from three day old seedlings;
2) chilling
the isolated apical shoot tips;
3) dissecting the apical shoot tips to
expose meristematic cells;
4) introducing a transforming agent into the
dissected apical shoot tips; and
5) regenerating a plant from shoots which
form on the meristematic cells.
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Claim 27
A transformed cotton plant produced by:
a) isolating an apical shoot tip from a three day old cotton seedling;
b) chilling the isolated apical shoot tip;
c) dissecting the apical shoot
tip to expose meristematic cells;
d) exposing the dissected meristematic
cells to a recombinant Agrobacterium comprising a gene conferring a
desired phenotypic trait to a plant; and
e) regenerating a transgenic
cotton plant from the shoots which form from the meristematic cells.
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