Cotton - Patents owned and patent applications filed by The Institute of Molecular Agrobiology (now owned by Temasek Life Sciences Laboratory Ltd)
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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AU
782198 B2
- Earliest priority - 11 June 1999
- Filed - 11 June 1999
- Granted - 7 July 2005
- Expected expiry - 10 June 2019
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Title - High-efficiency Agrobacterium-mediated
transformation of cotton using petiole explants
Claim 1
A method for producing a transgenic cotton plant comprising the steps of:
(a) obtaining cotton petiole explants,
(b) exposing the petiole
explants to a culture of Agrobacterium tumefaciens that harbors a
vector comprising an exogenous gene and a selectable marker, the
Agrobacterium being capable of effecting the stable transfer of the
exogenous gene and selection agent resistance gene to the genome of the cells of
the petiole explant,
(c) culturing the petiole explants in medium
containing plant hormones to induce callus formation,
(d) selecting
transformed callus that expresses the exogenous gene,
(e) culturing the
selected callus in suspension culture to induce formation of embryoids,
(f) regenerating the embryoids into whole transgenic cotton plants.
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Granted AU 782198 recites an A.
tumefaciens-mediated transformation method to produce transgenic cotton
plants using the petiole explant. Induction of callus formation requires "plant
hormones", the definition of which is unclear in the description. Dependent
claim 17 recites use of 2,4-dichlorophenoxacetic acid (2,4-D) and kinetin as
these plant hormones.
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Institute of Molecular Agrobiology (now owned by Temasek Life
Sciences Laboratory Ltd)
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WO
2000/77230 A1
- Earliest priority - 11 June 1999
- Filed - 11 June 1999
- OPI - 21 December 2000
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Title - High-efficiency
Agrobacterium-mediated transformation of cotton using petiole explants
Claim 1
A method for producing a transgenic cotton plant comprising the steps of:
(a) obtaining cotton petiole explants,
(b) exposing the petiole explants
to a culture of Agrobacterium tumefaciens that harbors a vector
comprising an exogenous gene and a selectable marker, the Agrobacterium
being capable of effecting the stable transfer of the exogenous gene and
selection agent resistance gene to the genome of the cells of the petiole
explant,
(c) culturing the petiole explants to induce callus
formation,
(d) selecting transformed callus that expresses the exogenous
gene,
(e) culturing the selected callus in suspension culture to induce
formation of embryoids,
(f) regenerating the embryoids into whole
transgenic cotton plants.
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Method for producing a transgenic cotton plant by exposing petiole explants
to A. tumefaciens carrying a vector having a gene of interest. This is
followed by induction of callus and embryoid formation and regeneration of a
whole plant.
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AU
777365 B2
- Earliest priority - 10 March 1999
- Filed - 10 March 1999
- Granted - 14 October 2004
- Expected expiry - 9 March 2019
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Title - Agrobacterium-mediated transformation of
cotton with novel explants
Claim 1
A method for producing a transgenic cotton plant comprising the steps of:
(a) obtaining cotton fibrous root explants,
(b) culturing the fibrous
root explants to induce callus formation,
(c) exposing root callus to a
culture of Agrobacterium tumefaciens that harbors a vector comprising
an exogenous gene and a selectable marker, the Agrobacterium being
capable of effecting the stable transfer of the exogenous gene and selection
agent resistance gene to the genome of the cells of the callus,
(d)
culturing the callus in the presence of the selection agent to which the
selection agent resistance gene confers resistance so as to select for
transformed cells,
(e) inducing somatic embryo formation in the selected
callus culture, and
(f) regenerating the induced somatic embryos into whole
transgenic cotton plants,
wherein the cotton fibrous root explants in step
(a) are obtained by growing cotton seedlings in the presence of multi-effect
triazole.
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Granted AU 777365 recites an A.
tumefaciens-mediated transformation method for producing a transgenic
cotton plant, where "multi-effect triazole" must be added in the growth medium
of the cotton seedlings to obtain fibrous root explants.
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WO
2000/53783 A1
- Earliest priority - 10 March 1999
- Filed - 10 March 1999
- OPI - 14 September 2000
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Title - Agrobacterium-mediated transformation of
cotton with novel explants
Claim 1
A method for producing a transgenic cotton plant comprising the steps of:
(a) obtaining cotton fibrous root explants,
(b) culturing the fibrous
root explants to induce callus formation,
(c) exposing root callus to a
culture of Agrobacterium tumefaciens that harbors a vector comprising an
exogenous gene and a selectable marker, the Agrobacterium being capable of
effecting the stable transfer of the exogenous gene and selection agent
resistance gene to the genome of the cells of the callus,
(d) culturing the
callus in the presence of the selection agent to which the selection agent
resistance gene confers resistance so as to select for transformed cells,
(e) inducing somatic embryo formation in the selected callus culture, and
(f) regenerating the induced somatic embryos into whole transgenic cotton
plants.
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| Remarks |
- National phase entries of WO 2000/77230 in China (CN1352692 A), Europe
(EP1194579 A1) and Japan (JP2003502050T) are pending.
- Other national phase entries of WO 2000/77230 include Brazil (BR9917361 A)
and Mexico (MXPA01012750 A).
- National phase entry of WO 2000/53783 in China (CN1240841) has been granted
on 8 February 2006.
- National phase entry of WO 2000/53783 in Europe (EP1159436 A1) is pending.
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Note: Patent information on this page was last updated on 15 March 2006.
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