Cotton - Patent application filed by Aventis CropScience (now Bayer BioScience NV)

A method for producing a transgenic cotton plant, comprising the step of

• incubating Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border with a plant phenolic compound capable of inducing increased vir gene expression in said Agrobacterium cells prior to or during the cocultivation of solid cotton embryogenic callus cultivated on solid media with said Agrobacterium cells.

A method for producing a transgenic cotton plant, said method comprising:

a) cocultivating cotton embryogenic callus with Agrobacterium cells, said Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border, in the presence of a plant phenolic compound capable of inducing increased vir gene expression in said Agrobacterium cells for a time sufficient to generate embryogenic callus comprising a transformed cotton cell; and
b) regenerating a transgenic cotton plant from said transformed cell.

A method for producing a transgenic cotton plant by Agrobacterium-mediated transformation, characterised in that

• Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border are co-cultivated with solid cotton embryogenic callus cultivated on solid media in the presence of a plant phenolic compound capable of inducing vir gene expression in said Agrobacterium cells.

Use of a plant phenolic compound capable of inducing increased vir gene expression in said Agrobacterium cells for Agrobacterium-mediated transformation of solid cotton embryogenic callus cultivated on solid media.

A method for producing a transgenic cotton plant comprising the steps of:

(A) incubating Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border, with a plant phenolic compound capable of inducing increased vir gene expression in said Agrobacterium cells;
(B) co-cultivating solid cotton embryogenic callus cultivated on solid media with said Agrobacterium cells to generate embryogenic callus comprising a transformed cotton cell; and
(C) regenerating a transgenic cotton plant from said transformed cell; wherein said incubating step occurs prior to or during said co-cultivation step.

A method for producing a transgenic cotton plant comprising the steps of:

(A) co-cultivating solid cotton embryogenic callus cultivated on solid media with Agrobacterium cells, said Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border, in the presence of a plant phenolic compound capable of inducing increased vir gene expression in said Agrobacterium cells, for a time sufficient to generate embryogenic callus comprising a transformed cotton cell; and
(B) regenerating a transgenic cotton plant from said transformed cell.

A process for producing a transgenic cotton plant comprising:

(A) co-cultivating solid cotton embryogenic callus cultivated on solid media, wherein said cotton embryogenic callus has not been generated from a cotton explant comprising transformed cells, with Agrobacterium cells in the presence of a plant phenolic compound capable of inducing increased vir gene expression in said Agrobacterium cells; said Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border; wherein said Agrobacterium cells are cocultivated with said cotton embryogenic callus for a time sufficient to generate embryogenic callus comprising a transformed cotton cell; and
(B) regenerating a transgenic cotton plant from said transformed cell.

A method for producing a transgenic cotton plant, comprising the step of:

• incubating Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border with a plant phenolic compound prior to or during the cocultivation of cotton embryogenic callus with said Agrobacterium cells.

A method for producing a transgenic cotton plant, said method comprising:

• cocultivating cotton embryogenic callus with Agrobacterium cells, said Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border, in the presence of a plant phenolic compound, for a time sufficient to generate embryogenic callus comprising a transformed cotton cell; and
• regenerating a transgenic cotton plant from said transformed cell.

A method for producing a transgenic cotton plant by Agrobacterium-mediated transformation comprising

• co-cultivating Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border with cotton embryogenic callus, characterized in that said cocultivating occurs in the presence of a plant phenolic compound.

Use of a plant phenolic compound for Agrobacterium-mediated transformation of cotton embryogenic callus.

A method for producing a transgenic cotton plant by Agrobacterium-mediated transformation characterized in that Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border are cocultivated with cotton embryogenic callus in the presence of a plant phenolic compound.