Summary
FreshWorld has a granted patent in the United States related
to a method for transforming plumule material or cotyledons of a pea seed,
either from a garden pea plant or an edible pod, with A. tumefaciens
or A. rhizogenes carrying a gene of interest. A related Australian
application was abandoned.
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5286635
- Earliest priority - 24 September 1991
- Filed - 24 September 1991
- Granted - 15 February 1994
- Expired - 15 March 2006
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Title - Genetically transformed pea plants and methods for
their production
| Claim 1
A method for genetically transforming a pea plant, said method comprising:
A) culturing explant material from the seed plumule of the pea plant with
Agrobacterium tumefaciens or rhizogenes cells
carrying an exogenous DNA sequence, wherein the strain of Agrobacterium
is selected to be capable of infecting and transferring DNA to the explant
material;
B) regenerating shoots from the explant material from step (A),
wherein said shoots are obtained from non-callus material;
C) selecting
regenerated shoots from step (B) which express the exogenous DNA sequence,
and
D) rooting said regenerated shoots to produce viable pea plants
expressing the exogenous DNA sequence.
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| Claim 7
A method for genetically transforming a pea plant, said method comprising:
A) obtaining plumule material from a pea plant seed;
B) culturing the
plumule material with Agrobacterium tumefaciens or rhizogenes
cells carrying an exogenous DNA sequence in a co-cultivation medium for a time
and under conditions selected to effect transfer of the exogenous DNA sequence
to cells of the plumule material, wherein the strain of Agrobacterium
is selected to be capable of infecting and transferring DNA to the explant
material;
C) separating the Agrobacterium cells form the plumule
material;
D) regenerating shoots from the plumule material from step (C) in
a regeneration medium for a time and under conditions selected to produce shoots
from non-callus regions of the plumule;
(E) selecting regenerated shoots
from step (D) which express the exogenous DNA sequence; and
(F) rooting the
selected regenerated shoots from step (E) in a rooting medium to produce plants
which express the exogenous DNA sequence.
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| Claim 19
A method for genetically transforming a pea plant, said method comprising:
A) preparing an explant by removing the seed coat from a sterilized seed of
the pea plant and cutting off the cotyledons where they are attached to the
embryo;
B) culturing explant material from sterilized seed of the pea plant
with Agrobacterium tumefaciens or rhizogenes cells
carrying an exogenous DNA sequence, wherein the strain of Agrobacterium
is selected to be capable of infecting and transferring DNA to the explant
material;
C) regenerating shoots from the explant material from step (A),
wherein said shoots are obtained from non-callus material;
D) selecting
regenerating shoots from step (B) which express the exogenous DNA sequence,
and
E) rooting said regenerated shoots to produce viable pea plants
expressing the exogenous DNA sequence.
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Granted US 5286635 has expired due to
non-payment of maintenance fees according to the USPTO.
Method for transforming the plumule of a pea seed and cotyledons with A.
tumefaciens or A. rhizogenes having an exogenous gene. Shoot
regeneration and production of viable pea plants expressing the gene of interest
are also claimed.
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FreshWorld
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WO
1993/06209 A1
- Earliest priority - 24 September 1991
- Filed - 9 September 1992
- OPI - 1 April 1993
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Title - Genetically transformed pea plants and methods for
their production
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Claim 1
A method for genetically transforming a pea plant, said method comprising:
(a) culturing explant material from the pea plant with
Agrobacterium cells carrying an exogenous DNA sequence;
(b)
regenerating shoots from the explant material from step (a), wherein said shoots
are obtained from non-callus material; and
(c) selecting regenerated shoots
from step (b) which express the exogenous DNA sequence.
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Claim 8
A method for genetically transforming a pea plant, said method comprising:
(a) culturing explant material from the seed plumule of the pea plant with
Agrobacterium cells carrying an exogenous DNA sequence;
(b)
regenerating shoots from the explant material from step (a); and
(c)
selecting regenerated shoots from step (b) which express the exogenous DNA
sequence.
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Claim 15
A method for genetically transforming a pea plant, said method comprising:
(a) obtaining a plumule material from pea plant seed;
(b) culturing the
plumule material with Agrobacterium cells carrying an exogenous DNA
sequence in a cocultivation medium for a time and under conditions selected to
effect transfer of the exogenous DNA sequence to cells of the plumule
material;
(c) separating the Agrobacterium cells from the plumule
material;
(d) regenerating shoots from the plumule material from step (c)
in a regeneration medium for a time and under conditions selected to produce
shoots from non-callus regions of the plumule;
(e) selecting regenerated
shoots from step (d) which express the exogenous DNA sequence; and
(f)
rooting the selected regenerated shoots from step (e) in a rooting medium.
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Claim 33
A pea plant which expresses a heterologous gene.
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Remarks
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National phase entry of WO 1993/06209 in Australia (AU
26441/92) has lapsed on 16 June 1994.
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Note: Patent information on this page was last updated on 21 March 2006.
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