Summary
The invention disclosed by Fillatti & Comai (owned by
Calgene Inc.) in patents granted in the United States and Australia is directed
to the transformation of poplar leaves with an armed (tumor-causing genes)
A. tumefaciens having a gene of interest.
The construct inserted into a transformed poplar comprises a transcription
initiation region, an open reading frame (ORF) expressing a peptide and a
transcription termination region.
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
4795855
- Earliest priority - 14 November 1985
- Filed - 24 February 1986
- Granted - 3 January 1989
- Expected expiry - 3 January 2006
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Title - Transformation and foreign gene expression with
woody species
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Claim 1
A transformed poplar plant comprising
transformed cells, said cells comprising a DNA construct as a result of
transforming of poplar cells with said DNA construct, which construct comprises
in the 5'-3' direction
(1) a transcription-initiation region;
(2) an open reading frame other than T-DNA expressing a peptide downstream
from said transcription-initiation region, and under the transciptional
regulation of said transcription-initiation region; and
(3) a transcription-termination region to provide an expression cassette
capable of expression in said cells, wherein
- at least one of said open reading frame, transcription initiation region,
and transcription termination region is from a plant other than poplar, and
wherein
- said expression cassette is bordered by at least the right T-DNA border, and
wherein
- said construct is a result of joining in vitro at least two of (1),
(2), and (3) and said right T-DNA border.
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Claim 7
Stably transformed poplar seedlings comprising
cells comprising a DNA construct as a result of transformation of poplar cells
with said DNA construct, which construct comprises in the 5'-3' direction:
(1) a transcription initiation region,
(2) an open reading frame, other than T-DNA, encoding a peptide, said open
reading frame downstream from said transcription initiation region and under the
transcriptional regulation of said transcription initiation region, and
(3) a transcription termination region, to provide an expression cassette
capable of expression in said poplar cells, wherein
- at least one of said open reading frame, transcription initiation region,
and transcription termination region is from a plant other than poplar, and
wherein
- said construct is as a result of joining in vitro at least two of
(1), (2), and (3).
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Claim 9
A method for transforming a poplar plant which
comprises:
(a) preincubating poplar leaf explants from shoot cultures with a medium
conditioned with plant cells;
(b) cocultivating said leaf explants with Agrobacterium tumefaciens
comprising an armed Ti-plasmid containing vir genes and an expression
construct comprising transcriptional initiation and termination regulatory
regions functional in said poplar and a gene other than the wild-type gene of
one or both of the initiation and termination regions and under their regulatory
control, bordered by at least the right T-DNA border, whereby said expression
construct becomes integrated into the genome of cells of said leaf explant;
(c) transferring said leaf explants after cocultivation to a regeneration
medium comprising plant hormones and phytohormones produced by a Ti-plasmid
comprising A. tumefaciens strain, whereby callus is formed and shoots
develop; and
(d) transferring shoots to growing medium to produce a poplar plant.
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Granted US 4795855 is a
continuation-in-part of abandoned US 06/798050.
Method to transform poplar leaf explants with A. tumefaciens having
a construct with a gene of interest flanked by a right T-DNA border.
Regeneration of transformed poplar plants from the explants, and elements of the
construct are also claimed.
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Calgene Inc.
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AU 597916 B2
- Earliest priority - 14 November 1985
- Filed - 13 November 1986
- Granted - 14 June 1990
- Expired - 9 September 1993
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Title - Transformation and foreign gene expression with
woody species e.g. Poplar
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Claim 1
A transformed poplar plant comprising
transformed cells, said cells comprising a DNA construct as a result of
transforming of poplar cells with said DNA construct, which construct comprises
in the 5'-3' direction
(1) a transcription-initiation region;
(2) an open reading frame other than T-DNA expressing a peptide downstream
from said transcription-initiation region, and under the transciptional
regulation of said transcription-initiation region; and
(3) a transcription-termination region to provide an expression cassette
capable of expression in said cells, wherein
- at least one of said open reading frame, transcription initiation region,
and transcription termination region is from a plant other than poplar, and
wherein
- said expression cassette is bordered by at least the right T-DNA border, and
wherein
- said construct is a result of joining in vitro at least two of (1),
(2), and (3) and said right T-DNA border.
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Claim 8
Stably transformed poplar seedlings comprising cells comprising a DNA
construct as a result of transformation of poplar cells with said DNA construct,
which construct comprises in the 5'-3' direction:
(1) a transcription initiation region,
(2) an open reading frame, other than T-DNA, encoding a peptide, said open
reading frame downstream from said transcription initiation region and under the
transcriptional regulation of said transcription initiation region, and
(3) a transcription termination region, to provide an expression cassette
capable of expression in said poplar cells, wherein
- at least one of said open reading frame, transcription initiation region,
and transcription termination region is from a plant other than poplar, and
wherein
- said construct is as a result of joining in vitro at least two of
(1), (2), and (3).
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Claim 10
A method for transforming a poplar plant which comprises:
(a) preincubating poplar leaf explants from shoot cultures with a medium
conditioned with plant cells;
(b) cocultivating said leaf explants with Agrobacterium tumefaciens
comprising an armed Ti-plasmid containing vir genes and an expression
construct comprising transcriptional initiation and termination regulatory
regions functional in said poplar and a gene other than the wild-type gene of
one or both of the initiation and termination regions and under their regulatory
control, bordered by at least the right T-DNA border, whereby said expression
construct becomes integrated into the genome of cells of said leaf explant;
(c) transferring said leaf explants after cocultivation to a regeneration
medium comprising plant hormones and phytohormones produced by a Ti-plasmid
comprising A. tumefaciens strain, whereby callus is formed and shoots
develop; and
(d) transferring shoots to growing medium to produce a poplar plant.
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Granted AU 597916 contains the same three independent claims as those of US
4795855 (see above).
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Remarks
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- Related application in Europe (EP 227264) is deemed to be withdrawn on 12
May 1993 as reported on INPADOC.
- Related patent granted in New Zealand (NZ 217915), which has lapsed on 14
January 1994 as reported by IPONZ.
- Other jurisdiction in which a related patent was filed includes Finland (FI
864331; application lapsed as reported by INPADOC).
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Note: Patent information on this page was last updated on 22 March 2006.
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