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WO
1992/00371 A1
- Earliest priority - 1 April 1991
- Filed - 21 June 1991
- OPI - 9 January 1992
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Title - Rose plants and methods for their production and
transformation
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Claim 1
A method for controlled regeneration of a rose plantlet from a somatic embryo
which comprises:
(a) providing a somatic embryo;
(b) culturing the somatic embryo on a
maturation medium capable of inducing differentiation of the embryo to yield a
differentiated embryo;
(c) germinating the differentiated embryo on
germination medium to yield a germinated embryo; and
(d) propagating the
germinated embryo on propagation medium to produce a mature plantlet capable of
being transferred to soil conditions.
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Claim 8
A method for obtaining at least one somatic embryo from mature somatic tissue
of rose plant which comprises:
(a) culturing mature somatic tissue on callus induction medium comprising
effective amounts of a nutrient medium, an energy source, an auxin and a
cytokinin to obtain at least one induced callus; and
(b) culturing the
induced callus in a regeneration media capable of inducing completion of the
development of somatic embryos comprising effective amounts of a nutrient
medium, an energy source, an auxin and a cytokinin in which the source of the
auxin and cytokinin in the regeneration media differs from the source of the
auxin and cytokinin in the callus induction medium to obtain at least one
somatic embryo.
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Claim 17
A method for obtaining a somatic embryo from a stamen filament of rose plant
which comprises:
(a) culturing the stamen filament on callus induction medium comprising
effective amounts of a nutrient medium, an energy source, an auxin and a
cytokinin to obtain at least one induced callus; and
(b) culturing the
induced callus in a regeneration media capable of inducing completion of the
development of somatic embryos comprising effective amounts of a nutrient
medium, an energy source, an auxin and a cytokinin in which the ratio of auxin
to cytokinin is decreased by a factor of two to about 15 relative to the ratio
of auxin to cytokinin in the callus induction medium to obtain a somatic embryo.
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Claim 20
A method for obtaining a somatic embryo from a leaf explant of rose plant
which comprises:
(a) culturing the leaf explant on a callus induction medium comprising
effective amounts of a nutrient medium, an energy source, an auxin and a
cytokinin to obtain at least one induced callus; and
(b) culturing the
induced callus in a regeneration media capable of inducing completion of the
development of somatic embryos comprising effective amounts of a nutrient
medium, an energy source, an auxin and a cytokinin to which the source of the
auxin and cytokinin in the regeneration media differs from the source of the
auxin and cytokinin in the callus induction medium to obtain a somatic embryo.
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Claim 26
A method for genetically transforming callus cells from a rose plant, said
method comprising:
- incubating the callus cells with Agrobacterium cells carrying an
exogenous DNA sequence; and
- selecting callus cells which express at least a portion of the exogenous DNA
sequence.
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Claim 29
A method for genetically transforming a rose plant, said method comprising:
(a) culturing tissue from the rose plant under conditions selected to produce
a callus;
(b) incubating cells from the callus of step (a) with
Agrobacterium cells carrying an exogenous DNA sequence;
(c)
selecting callus cells from step (b) which express at least a portion of the DNA
sequence; and
(d) producing transformed plantlets from the selected callus
cells of step (c).
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Claim 34
A method for producing a somatic rose embryo which expresses an exogenous DNA
sequence, said method comprising:
(a) culturing tissue from a rose plant on a callus induction medium
containing nutrients, an energy source, an auxin, and growth regulator, a
cytokinin in amounts effective to induce callus formation wherein the tissue is
derived from a plant part selected from the group consisting of stamen
filaments, leaf explants, stem sections, shoot tips, petal, sepal, petiole, and
peduncle;
(b) combining cells from the callus of step (a) with
Agrobacterium cells carrying the exogenous DNA sequence in a
cocultivation medium containing nutrients, an energy source, and an induction
compound under conditions which allow the Agrobacterium cells to infect
the callus cells and transfer the exogenous DNA sequence to the callus cell
chromosomes;;
(c) culturing callus cells from step (b) in a selection
medium containing nutrients, an energy source, an auxin, a cytokinin, and an
agent which inhibits the growth of callus cells which do not express the
selectable marker gene; and
(d) culturing the cells selected in step (c)
in a regeneration medium containing nutrients, an energy source, an
antibacterial agent, and a growth regulator selected from abscisic acid and
giberellic acid, other than an auxin or a cytokinin, present in amounts
effective to produce somatic embryos.
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Claim 50
A rose callus cell which expresses an exogenous DNA sequence.
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Claim 51
A rose plant having cells which express an exogenous DNA sequence.
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Claim 52
A somatic rose embryo which expresses an exogenous DNA sequence.
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The PCT application has DNA Plant Technology Co. as a
co-assignee listed with Florigene B.V..
The claims of the PCT application recite methods to regenerate a rose
plantlet from a somatic embryo and to obtain a somatic embryo from somatic rose
tissue, i.e. stamen filament, and leaf. Similar to its related United States
patent, methods are also claimed to transform a rose callus with
Agrobacterium having an exogenous gene, to produce a transformed
somatic embryo and transformed rose plantlets.
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Florigene B.V.
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US
2001/007157 A1
- Earliest priority - 18 November 1993
- Filed - 10 August 1998
- Abandoned - 15 July 2002
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Title - Genetically transformed rose plants and methods for
their production
Claim 1
A method for genetically transforming callus cells from a rose plant, said
method comprising:
- incubating the callus cells with Agrobacterium cells carrying an
exogenous DNA sequence; and
- selecting callus cells which express at least a portion of the exogenous DNA
sequence.
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Claim 6
A method for genetically transforming a rose plant, said method comprising:
(a) culturing tissue from the rose plant under conditions selected to produce
a callus;
(b) incubating cells from the callus of step (a) with
Agrobacterium cells carrying an exogenous DNA sequence;
(c)
selecting callus cells from step (b) which express at least a portion of the DNA
sequence; and
(d) producing transformed plantlets from the selected callus
cells of step (c).
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Claim 13
A method for producing a somatic rose embryo which expresses an exogenous DNA
sequence including a selectable marker gene, said method comprising:
(a) culturing tissue from a rose plant on a callus induction medium
containing nutrients, an energy source, an auxin, and a cytokinin in amounts
effective to induce callus formation;
(b) combining cells from the callus
of step (a) with Agrobacterium cells carrying the exogenous DNA
sequence in a cocultivation medium containing nutrients, an energy source, and
an induction compound under conditions which allow the Agrobacterium
cells to infect the callus cells and transfer the exogenous DNA sequence to the
callus cell chromosomes;
(c) culturing callus cells from step (b) in a
selection medium containing nutrients, an energy source, an auxin, a cytokinin,
and an agent which inhibits the growth of callus cells which do not express the
selectable marker gene; and
(d) culturing the cells selected in step (c)
in a maintenance medium containing nutrients, an energy source, an antibacterial
agent, and a growth regulator, other than an auxin or a cytokinin, present in
amounts effective to produce somatic embryos.
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Claim 38
A rose callus cell which expresses an exogenous DNA sequence.
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Claim 39
A rose plant having cells which express an exogenous DNA sequence.
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Claim 40
A somatic rose embryo which expresses an exogenous DNA sequence.
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Abandoned application US 2001/007157 is a
continuation of now granted US 5792927, which
is a divisional of now granted US 5480789
(see above).
Methods for transforming a rose callus with Agrobacterium cells
carrying an exogenous DNA. Unlike the granted patent US
5792927, the type of callus to be transformed is not specified.
A
method for producing a somatic rose embryo expressing an exogenous sequence is
also described. Rose callus, somatic embryo and plant expressing an exogenous
gene are recited in the filed claims.
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