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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5416011
- Earliest priority - 22 July 1988
- Filed - 23 November 1993
- Granted - 16 May 1995
- Expected expiry - 16 May 2012
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Title - Method for soybean transformation and regeneration
| Claim 1
A method for transforming soybeans which comprises:
A) preparing a cotyledon explant from a soybean seedling by:
(i)
removing the hypocotyl region by cutting just below the cotyledonary node,
(ii) separating the two cotyledons at the cotyledonary node by tearing the
cotyledons apart, and
(iii) removing the epicotyl from the cotyledon to
which it remains attached, B) inserting a chimeric gene into the explant of part
(A) by inoculation of at least the region adjacent to the axillary bud of the
explant with a disarmed Agrobacterium tumefaciens vector containing
said chimeric gene;
C) selecting transformed explant tissue, and
D)
regenerating a differentiated transformed plant from the transformed explant
tissue of part (C).
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| Claim 10
A method for transforming soybeans which comprises:
A) preparing a cotyledon explant from a soybean seedling by:
(i)
removing the hypocotyl region by cutting just below the cotyledonary node,
(ii) separating the two cotyledons at the cotyledonary node by tearing the
cotyledons apart, and
(iii) removing the epicotyl from the cotyledon to
which it is attached, and
(iv) wounding the explant by making at least one
cut in the axillary bud region of the explant, B) inserting a chimeric gene into
the explant of part (A) which gene encodes for neomycin phosphotransferase II by
inoculation and co-cultivation of the explant with a disarmed Agrobacterium
tumefaciens vector containing said chimeric gene;
C) selecting
transformed explant tissue by growing the explant in the presence of kanamycin,
and
D) regenerating a differentiated transformed plant from the transformed
explant tissue of part (C).
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| Claim 12
A method for transforming soybeans which comprises:
A) preparing a cotyledon explant from a soybean seedling by:
(i)
removing the hypocotyl region by cutting just below the cotyledonary node,
(ii) separating the two cotyledons at the cotyledonary node, and
(iii) removing the epicotyl from the cotyledon to which it remains attached, B)
inserting a chimeric gene into the explant of part (A) by inoculation of at
least the region adjacent to the axillary bud of the explant with a disarmed
Agrobacterium tumefaciens vector containing said chimeric gene;
C) selecting transformed explant tissue, and
D) regenerating a
differentiated transformed plant from the transformed explant tissue of part
(C).
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Transformation of a cotyledon of a soybean seedling by inoculation with a
disarmed A. tumefaciens vector having a chimeric gene. As part of the
invention, the chimeric gene encodes for npt II.
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Monsanto
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US
5569834
- Earliest priority - 22 July 1988
- Filed - 14 February 1995
- Granted - 29 October 1996
- Expected expiry - 16 May 2012
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Title - Method for soybean transformation and regeneration
| Claim 1
A soybean plant comprising a chimeric gene and associated DNA resulting from
an Agrobacterium tumefaciens -mediated transformation, said chimeric
gene capable of conferring kanamycin resistance to said soybean plant, produced
by the method which comprises:
A) preparing a cotyledon explant from a soybean seedling by:
(i)
removing the hypocotyl region by cutting just below the cotyledonary node,
(ii) separating the two cotyledons at the cotyledonary node by tearing the
cotyledons apart, and
(iii) removing the epicotyl from the cotyledon to
which it remains attached, B) inserting a chimeric gene into the explant of part
(A) by inoculation and co-cultivation of the explant with a disarmed
Agrobacterium tumefaciens vector containing said chimeric gene;
C) selecting transformed explant tissue, and
D) regenerating a
differentiated transformed plant from the transformed explant tissue of part
(C).
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United States granted patent US 5569834 is a
divisional of now granted US 5416011 (see
above).
Method to obtain a soybean plant resistant to kanamycin via A.
tumefaciens transformation. The explant for transformation is a cotyledon
isolated from a soybean seedling.
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US
5824877
- Earliest priority - 22 July 1988
- Filed - 25 October 1996
- Granted - 20 October 1998
- Expected expiry -
- 16 May 2012
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Title - Method for soybean transformation and regeneration
| Claim 1
A method for transforming soybean which comprises:
A) preparing a cotyledon explant from a soybean seedling by:
(i)
removing the hypocotyl region by cutting just below the cotyledonary node,
(ii) separating the two cotyledons at the cotyledonary node by tearing the
cotyledons apart, and
(iii) removing the epicotyl from the cotyledon to
which it remains attached, B) inserting a chimeric gene into the explant of part
(A) by inoculation and co-cultivation of the explant with a disarmed
Agrobacterium tumefaciens vector containing said chimeric gene;
C) selecting transformed explant tissue, and
D) regenerating a
differentiated transformed plant from the transformed explant tissue of part
(C).
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| Claim 10
A method for transforming soybean which comprises:
A) preparing a cotyledon explant from a soybean seedling by:
(i)
removing the hypocotyl region by cutting just below the cotyledonary node,
(ii) separating the two cotyledons at the cotyledonary node by tearing the
cotyledons apart, and
(iii) removing the epicotyl from the cotyledon to
which it is attached, and
(iv) wounding the explant by making at least one
cut in the axillary bud region of the explant, B) inserting a chimeric gene into
the explant of part (A) which gene encodes for 5-enolpyruvylshikimatephosphate
synthase by inoculation and co-cultivation of the explant with a disarmed
Agrobacterium tumefaciens vector containing said chimeric gene;
C) selecting transformed explant tissue by growing the explant in the presence
of glyphosate, and
D) regenerating a differentiated transformed plant from
the transformed explant tissue of part (C).
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United States granted patent US 5824877 is a
divisional of now granted US 5569834 (see
above).
As in the previous United States patents, a cotyledon explant is transformed
by inoculation and co-cultivation with A. tumefaciens containing a gene
encoding glyphosate resistance.
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US
5959179
- Earliest priority - 13 March 1996
- Filed - 13 March 1996
- Granted - 28 September 1999
- Expected expiry - 13 March 2016
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Title - Method for transforming soybeans
| Claim 1
A method for transforming soybean, comprising:
A) preparing a cotyledon explant from a soybean seedling by:
(i)
incubating said seedling at about 0ºC to about 10ºC for at least 24 hours;
(ii) removing the hypocotyl region by cutting in the region of from about
0.2 to about 1.5 cm below the cotyledonary node;
(iii) splitting and
completely separating the remaining attached hypocotyl segment, also thereby
separating the two cotyledons;
(iv) removing the epicotyl from the
cotyledon to which it remains attached; and
(v) wounding the cotyledon in
the region of the axillary bud; B) inserting DNA to be introduced into said
explant of step (a) by inoculating at least the region adjacent to the axillary
bud of the explant with a disarmed Agrobacterium tumefaciens vector
containing said DNA;
C) selecting transformed explant tissue; and
D)
regenerating a differentiated transformed plant from said transformed explant
tissue of step (c).
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A method for transforming a soybean cotyledon by inoculating into the
axillary bud region a disarmed A. tumefaciens vector containing DNA of
interest.
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WO
1998/00557 A2
- Earliest priority - 28 June 1996
- Filed - 28 May 1997
- OPI - 8 January 1998
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Title - Methods of optimizing substrate pools and
biosynthesis of poly-'beta'-hydroxybutyrate-co-poly-'beta'-hydroxyvalerate in
bacteria and plants
Claim 37
A method for transforming soybean, comprising:
(a) preparing a cotyledon
explant from a soybean seedling by:
(i) incubating said seedling at about
0˚C to about 10˚C for at least 24 hours;
(ii) removing the hypocotyl
region by cutting in the region of from about 0.2 to about 1.5 cm below the
cotyledonary node;
(ii) splitting and completely separating the remaining
attached hypocotyl segment, also thereby separating the two cotyledons;
(iii) removing the epicotyl from the cotyledon to which it remains attached; and
(iv) wounding the cotyledon in the region of said axillary bud;
(b)
inserting DNA to be introduced into said explant of step (a) by inoculating at
least the region adjacent to the axillary bud of the explant with a disarmed
Agrobacterium tumefaciens vector containing said DNA;
(c)
selecting transformed explant tissue; and
(d) regenerating a differentiated
transformed plant from said transformed explant tissue of step (c).
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PCT application WO 1998/00557 recites genes and methods for
optimizing levels of substrates employed in the biosynthesis of copolymers of
3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria using
recombinant DNA technology. Claim 37 is one of the independent claims that
recite a method to transform soybean using a disarmed A. tumefaciens
containing DNA that is to be introduced.
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US
6384301
- Earliest priority - 14 January 1999
- Filed - 20 December 2001
- Granted - 24 October 2002
- Expected expiry - 20 December 2021
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Title - Soybean Agrobacterium transformation method
Claim 1
A method of making germline-transformed soybean plants using
Agrobacterium mediation, the method comprising:
(a) initiating the germination of a soybean seed;
(b) isolating the
embryonic axis including the embryonic meristem from the soybean seed to prepare
an explant;
(c) wounding the explant;
(d) exposing the explant to a
disarmed Agrobacterium vector comprising a heterologous genetic
construct comprising a selectable marker gene wherein the heterologous genetic
construct is transferred into at least one cell in the explant;
(e)
culturing the explant in the presence of a selection agent in a manner allowing
identification of soybean cells of the explant to which the heterologous genetic
construct has been transferred;
(f) inducing formation of one or more
shoots from the explant, the shoot comprising germline transformed cells;
(g) cultivating the shoot into a whole fertile mature soybean plant.
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A method for soybean transformation using a wounded embryonic axis isolated
from germinating seed as a target for Agrobacterium-mediated
transformation. The explant is cultured in a medium containing a selection
agent.
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US
7002058
- Earliest priority - 14 January 1999
- Filed - 20 December 2001
- Granted - 21 February 2006
- Expected expiry - 20 December 2021
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Title - Soybean transformation method
Claim 1
A method of making germline-transformed soybean plants using
Agrobacterium mediation, the method comprising:
(a) initiating the germination of a soybean seed;
(b) isolating the
embryonic axis including the embryonic meristem from the soybean seed to prepare
an explant;
(c) exposing the explant to a disarmed Agrobacterium
vector comprising a heterologous genetic construct comprising a selectable
marker gene wherein the heterologous genetic construct is transferred into at
least one cell in the explant;
(d) culturing the explant in the presence
of a selection agent in a manner allowing identification of soybean cells of the
explant to which the heterologous genetic construct has been transferred;
(e) inducing formation of one or more shoots from the explant, the shoot
comprising germline transformed cells;
(f) cultivating the shoot into a
whole fertile mature soybean plant.
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Claim 11
A method of transforming a soybean cell using Agrobacterium
comprising:
(a) initiating the germination of a soybean seed;
(b) isolating the
embryonic axis including the embryonic meristem from the soybean seed to prepare
an explant;
(c) exposing the explant to a disarmed Agrobacterium
vector comprising a heterologous genetic construct comprising a selectable
marker gene wherein the heterologous genetic construct is transferred into at
least one cell in the explant;
(d) culturing the explant in the presence
of a selection agent in a manner allowing identification of soybean cells of the
explant to which the heterologous genetic construct has been transferred.
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United States granted patent US 7002058 is a
continuation of now granted US 6384301.
An Agrobacterium-mediated transformation method of soybean, where
the embryonic axis does not require wounding before it is exposed to a disarmed
Agrobacterium in comparison to claim 1 of US 6384301
(see above), where wounding of the embryogenic axis is included in the
transformation step.
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US
2006/0059589 A1
- Earliest priority - 14 January 1999
- Filed - 4 November 2005
- Application pending
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Title - Soybean transformation method
Claim 11
A method of producing a transformed soybean cell comprising:
(a) initiating the germination of a soybean seed;
(b) substantially
removing at least one cotyledon from the germinating seed;
(c) exposing at
least a portion of the meristematic region in the germinating seed;
(d)
exposing one or more cells in the meristematic region to a disarmed
Agrobacterium vector comprising a heterologous genetic construct,
wherein said genetic construct comprises a selectable marker gene, and said
genetic construct is transferred into at least one cell in the meristematic
region;
(e) culturing the cells of the meristematic region in the presence
of a selection agent to identify a transformed soybean cell that contains the
genetic construct.
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Claim 25
A method of producing a fertile transgenic soybean plant comprising:
(a) initiating the germination of a soybean seed;
(b) substantially
removing at least one cotyledon from the germinating seed;
(c) exposing at
least a portion of the meristematic region in the germinating seed;
(d)
exposing one or more cells in the meristematic region to a disarmed
Agrobacterium vector comprising a heterologous genetic construct,
wherein said genetic construct comprises a selectable marker gene, and said
genetic construct is transferred into at least one cell in the meristematic
region;
(e) culturing the cells of the meristematic region in the presence
of a selection agent in a manner that identifies a transformed soybean cell that
contains the genetic construct;
(f) inducing formation of one or more
shoots from the meristematic region, wherein said shoot comprises at least one
transformed cell, to produce a transgenic soybean shoot;
(g) cultivating
the transgenic soybean shoot into a fertile transgenic soybean plant.
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United States patent application US 2006/0059589 is a
divisional of now granted US 7002058 (see
above).
Claims 1-10 in this application have been cancelled according to the
publication.
This application recites an Agrobacterium-mediated transformation
method of soybean, where cells in the meristematic tissue are exposed to a
disarmed Agrobacterium.
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WO
2000/42207 A2
- Earliest priority - 4 November 1999
- Filed - 12 January 2000
- OPI - 20 July 2000
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Title - Soybean transformation method
Claim 1
A method of preparing a germline-transformed soybean plant using
Agrobacterium mediation, the method comprising:
(a) initiating
the germination of a soybean seed;
(b) isolating the embryonic axis from
the soybean seed to prepare an explant;
(c) wounding the explant;
(d) exposing the explant to a disarmed Agrobacterium vector
comprising a heterologous genetic construct including a selectable marker gene
under conditions in which the heterologous genetic construct is transferred into
at least one cell in the explant;
(e) culturing the explant in the
presence of a selection agent in a manner capable of identifying soybean cells
of the explant to which the heterologous genetic construct has been
transferred;
(f) inducing formation of one or more shoots from the
explant, the shoot comprising germline transformed cells;
(g) cultivating
the shoot into a whole fertile mature soybean plant.
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PCT application WO 2000/42207 recites an
Agrobacterium-mediated transformation method of soybean, where a
wounded embryonic axis is exposed to a disarmed Agrobacterium.
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| Remarks |
- National phase entry of WO 1998/00557 in Australia (AU 31443/97) has lapsed
on 25 March 1999.
- National phase entry of WO 1998/00557 in Canada (CA 2259251) is pending.
- National phase entry of WO 2000/42207 in Australia (AU 2000/28488) has
lapsed on 18 August 2005.
- National phase entry of WO 2000/42207 in Canada (CA 2359868) has lapsed on
12 January 2004.
- National phase entry of WO 2000/42207 in Europe (EP 1141346) is deemed to be
withdrawn on 3 March 2004.
- National phase entry of WO 2000/42207 in Japan (JP 2002534129) is pending.
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