Summary
The United States patent granted to Asgrow Seeds refers to
- a method for transforming squash embryogenic calli with
Agrobacterium,
- regeneration of transformed squash plants, and
- components of an induction medium for regeneration of the transformed calli.
Asgrow Seeds was previously a subsidiary of the Upjohn Company, which was
sold to Empresas La Moderna (ELM; Mexico) in 1994, and soon after became Seminis
Inc.. Seminis is now owned by Monsanto Co. (acquisition signed in 2005).
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5677157
- Earliest priority - 20 September 1989
- Filed - 5 December 1994
- Granted - 14 October 1997
- Expired - 16 November 2005
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Title - Somatic embryogenesis and transformation of squash
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Claim 1
A method of transforming and regenerating squash plants, which comprises:
A) excising shoot tips from germinating squash;
B) transforming
embryogenic calli by inoculating the excised squash tissue with
Agrobacterium comprising a DNA construct having a beneficial gene and a
plant expressible selection marker gene and culturing the resulting explant on
an induction media comprising MS media, 2,4,5-T, BAP, and Kn;
C)
selectively growing the transformed embryogenic calli on media containing a
selection agent for the plant expressible selection marker gene; and
D)
subjecting the transformed embryogenic calli to an embryogenic regeneration
procedure from which whole transformed squash plants can be obtained.
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Claim 4
A method of transforming and regenerating squash plants, which comprises:
A) excising tissue from mature squash seeds;
B) transforming embryogenic
calli by inoculating said excised squash tissue with Agrobacterium
comprising a DNA construct having a beneficial gene and a plant expressible
selection marker gene and culturing on an induction media comprising MS media,
2,4-D or 2,4,5-T, BAP, and Kn;
C) selectively growing the transformed
embryogenic calli on media containing a selection agent for the plant
expressible selection marker gene; and
D) subjecting the transformed
embryogenic calli to an embryogenic regeneration procedure from which whole
transformed squash plants can be obtained.
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Granted US 5677157 has expired due to
non-payment of maintenance fees.
Methods to transform embryogenic calli derived from squash shoot tips and
squash seeds with Agrobacterium having a gene of interest. Media for
culturing the transformed explant and embryogenic regeneration procedure are
claimed.
Additionally, transformation of the same explants by microprojectile
bombardment is also claimed. The claims referring to this transformation method
are not shown here.
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Asgrow Seed Company
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WO
1991/04332 A1
- Earliest priority - 20 September 1989
- Filed - 22 August 1990
- OPI - 4 April 1991
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Title - Somatic embryogenesis of squash
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Claim 1
A method of regenerating and transforming Cucurbita pepo L. (squash)
plants, which belong to the family Cucurbitaceae, which comprises
(1) excising squash tissue selected from the group consisting of shoot tips
from germinating squash seeds and squash tissue from mature seeds,
(3)
producing embryogenic calli from said tissues, being either non-transformed or
transformed,
(4) selectively growing the transformed embryogenic call on
media containing kanamycin, and
(5) subjecting the transformed embryogenic
calli to an embryogenic regeneration procedure from which whole transformed
squash plants can be obtained.
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PCT application WO 1991/04332 recites a transformation and
regeneration method of Cucurbita pepo. Note that the steps in the
published document have skipped number 2. Agrobacterium-mediated
transformation of C. pepo is recited in dependent claim 7.
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The Upjohn Company
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Remarks
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- National phase entry of WO 1991/04332 in Australia (AU 62840/90) has lapsed
on 11 June 1992.
- National phase entry of WO 1991/04332 in Europe (EP 62840) has been granted
on 30 November 1994. Independent claim 1 does not limit the method of
transformation to that of Agrobacterium.
- National phase entry of WO 1991/04332 in Japan (JP H 05/500308) has been
deemed to be withdrawn on 25 November 1997.
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Note: Patent information on this page was last updated on 27 March 2006.
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