Sugar beet - Patent applications filed by
Monsanto
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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WO
2001/42480 A2
- Earliest priority - 7 December 1999
- Filed - 6 December 2000
- OPI - 14 June 2001
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Title - Sugarbeet regeneration and transformation
| Claim 1
A method for the preparation of transgenic sugarbeet cells, the method
comprising:
A) selecting a sugarbeet seedling, the seedling comprising a cotyledon region
and a hypocotyl region;
B) removing the cotyledon region and upper half of
the hypocotyl region from the seedling;
C) contacting the cotyledon region
and upper half of the hypocotyl region with micropropagation media to form a
micropropagated shoot, the micropropagated shoot comprising at least one leaf or
portion thereof comprising a leaf base;
D) removing a leaf from the
micropropagated shoot at the leaf base; and
E) contacting the leaf at the
leaf base with Agrobacteria in a manner forming a tissue comprising a transgenic
sugarbeet cell, capable of expressing an exogenous structural nucleic acid
sequence wherein:
the Agrobacteria comprises a vector; and
the vector
comprises operatively linked in the 5' to 3' orientation:
- a promoter that directs transcription of an exogenous structural nucleic
acid sequence;
- an exogenous structural nucleic acid sequence; and
- a 3' transcription terminator.
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| Claim 7
A method for the preparation of a transgenic sugarbeet plant, the method
comprising:
A) selecting a sugarbeet seedling, the seedling comprising a cotyledon region
and a hypocotyl region;
B) removing the cotyledon region and upper half of
the hypocotyl region from the seedling;
C) contacting the cotyledon region
and upper half of the hypocotyl region with micropropagation media to form a
micropropagated shoot, the micropropagated shoot comprising at least one leaf or
portion thereof comprising a leaf base;
D) removing a leaf from the
micropropagated shoot at the leaf base;
E) contacting the leaf base with
Agrobacteria in a manner forming a tissue comprising a transgenic sugarbeet
cell;
F) culturing said tissue to form a transgenic shoot;
G)
culturing the transgenic shoot to form a transgenic rooted shoot; and
H)
growing the transgenic rooted shoot to form a transgenic sugarbeet plant capable
of expressing an exogenous structural nucleic acid sequence, wherein:
the Agrobacteria comprise a vector; and
the vector comprises operatively
linked in the 5' to 3' orientation:
- a promoter that directs transcription of an exogenous structural nucleic
acid sequence;
- an exogenous structural nucleic acid sequence; and
- a 3' transcription terminator.
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A method for preparing transgenic sugar beet cells by selecting part of the
cotyledon and hypocotyl region of a sugar beet seedling and micropropagating
this part to form a shoot, from which a leaf is selected and put in contact with
Agrobacterium cells. The Agrobacterium cells contain a vector
with an exogenous gene. Transgenic sugar beet plants capable of expressing the
exogenous gene are also recited in the claims.
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Monsanto
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US
2001/42257 A1
- Earliest priority - 7 December 1999
- Filed - 6 December 2000
- Abandoned - 18 March 2004
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Title - Sugarbeet regeneration and transformation
| Claim 1
A method for the preparation of transgenic sugarbeet cells, the method
comprising:
A) selecting a sugarbeet seedling, the seedling comprising a cotyledon region
and a hypocotyl region;
B) removing the cotyledon region and upper half of
the hypocotyl region from the seedling;
C) contacting the cotyledon region
and upper half of the hypocotyl region with micropropagation media to form a
micropropagated shoot, the micropropagated shoot comprising at least one leaf or
portion thereof comprising a leaf base;
D) removing a leaf from the
micropropagated shoot at the leaf base; and
E) contacting the leaf at the
leaf base with Agrobacteria in a manner forming a tissue comprising a transgenic
sugarbeet cell, capable of expressing an exogenous structural nucleic acid
sequence wherein:
the Agrobacteria comprises a vector; and
the vector
comprises operatively linked in the 5' to 3' orientation:
- a promoter that directs transcription of an exogenous structural nucleic
acid sequence;
- an exogenous structural nucleic acid sequence; and
- a 3' transcription terminator.
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| Claim 7
A method for the preparation of a transgenic sugarbeet plant, the method
comprising:
A) selecting a sugarbeet seedling, the seedling comprising a cotyledon region
and a hypocotyl region;
B) removing the cotyledon region and upper half of
the hypocotyl region from the seedling;
C) contacting the cotyledon region
and upper half of the hypocotyl region with micropropagation media to form a
micropropagated shoot, the micropropagated shoot comprising at least one leaf or
portion thereof comprising a leaf base;
D) removing a leaf from the
micropropagated shoot at the leaf base;
E) contacting the leaf base with
Agrobacteria in a manner forming a tissue comprising a transgenic sugarbeet
cell;
F) culturing said tissue to form a transgenic shoot;
G)
culturing the transgenic shoot to form a transgenic rooted shoot; and
H)
growing the transgenic rooted shoot to form a transgenic sugarbeet plant capable
of expressing an exogenous structural nucleic acid sequence, wherein:
the Agrobacteria comprise a vector; and
the vector comprises operatively
linked in the 5' to 3' orientation:
- a promoter that directs transcription of an exogenous structural nucleic
acid sequence;
- an exogenous structural nucleic acid sequence; and
- a 3' transcription terminator.
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According to the USPTO, this application has been abandoned due to failure of
the applicant to respond to an office action. There are no continuity data
reported as yet.
The filed claims are worded the same as the claims of the related PCT patent
application.
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| Remarks |
- National phase entry of WO 2001/42480 in Australia (AU
2001/25757) and Canada (CA 2395365) have both
lapsed (AU 2001/25757 lapsed on 15 August 2002; CA 2395365
lapsed on 6 December 2004).
- Other national phase entries of WO 2001/42480 include Czech Republic
(CZ 20022139), Poland (PL 356025), Slovakia
(SK 200200804),
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Note: Patent information on this page was last updated on 28 March 2006.
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