Turnip rape (Brassica rapa) - Granted
patents assigned to the University of Helsinki
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6455761
- Earliest priority - 18 September 1997
- Filed - 10 March 2000
- Granted - 24 September 2002
- Expected expiry - 15 September 2018
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Title - Agrobacterium-mediated transformation of
turnip rape
Claim 1
A method for transforming mature plants of turnip rape, comprising
(i) excising an internode section of the inflorescence-carrying stem of a
mature turnip rape plant,
(ii) sterilizing said internode section, and
cutting it in 4-8 mm segments to obtain an internode segment,
(iii) placing
an internode segment in a horizontal position on an agar pre-cultivation medium
supplemented with 15-90 μM of silver nitrate and 2,4-dichlorophenoxyacetic acid
(2,4-D) hormone,
(iv) pre-cultivating the internode segment on said medium
for 1 day,
(v) immersing the internode segment in a MS solution inoculated
with Agrobacterium tumefaciens bacteria carrying at least one gene
heterologous to said turnip rape,
(vi) placing the immersed internode
segment in a horizontal position on an agar MS co-cultivation medium,
(vii) co-cultivating the internode segment with Agrobacteria for 2
days,
(viii) washing the internode segment to remove the
Agrobacteria,
(ix) placing the internode segment in a vertical
position with the basal side down on MS agar medium for selection with an
antibiotic, the medium being supplemented with cytokinine hormones and silver
nitrate, to obtain an internode segment with regenerated primordia or embryonic
green nodules,
(x) placing the internode segment with regenerated primordia
or embryogenic green nodules on a hormone-free regeneration medium, and
(xi) recovering the transgenic shoots regenerated.
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The independent claim of United Stated patent US 6455761
discloses a quite detailed protocol of how to obtain transgenic turnip rape
(Brassica rapa). This offers a number of opportunities to develop
similar methods and avoid infringement with respect to this specific patent at
the same time. Other patents have more general claims that cover the use of
Agrobacterium to produce transgenic dicots for example.
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University of Helsinki
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AU
732372 B2
- Earliest priority - 18 September 1997
- Filed - 16 September 1998
- Granted - 26 April 2001
- Patent ceased - 22 April 2004
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Title - Agrobacterium-mediated transformation of
turnip rape
Claim 1
A method for transformation of mature plants of turnip rape, comprising
- excising an internode section of the inflorescence carrying stem of a
mature turnip rape plant,
- sterilizing said internode section, and cutting
it in 4-8 mm segments,
- placing a segment in a horizontal position on an
agar pre-cultivation medium supplemented with silver nitrate and 2,
4-dichlorophenoxyacetic acid (2, 4-D) hormone,
- pre-cultivating the
segment on said medium for 1 day,
- immersing the segment in a MS solution
inoculated with Agrobacterium tumefaciens bacteria carrying at least
one gene heterologous to said turnip rape,
- placing the immersed segment
in a horizontal position on an agar MS co-cultivation medium,
-co-cultivating the segment with Agrobacteria for 2 days,
-
washing the segment from the Agrobacteria,
-placing the segment in
a vertical position with the basal side down on MS agar medium for selection
with an antibiotic, the medium being supplemented with cytokinine hormones and
silver nitrate, and
-placing the segment with regenerated primordia or
embryogenic green nodules on a hormone free regeneration medium, and
-
recovering the transgenic shoots regenerated.
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| Remarks |
- National phase entry of
WO
1999/14349 in Canada (CA 2302835) is deemed dead on 16 September 2004.
- National phase entry of
WO
1999/14349 in Europe (EP 1009845) is deemed to be withdrawn on 6 October
2004.
- Other national phase entry of
WO
1999/14349 includes Finland (FI 973720).
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Note: Patent information on this page was last updated on 13 March 2006.
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