Chrysanthemums
Summary
A transformed chrysanthemum plant
capable of expressing a gene of interest is the subject of the invention granted
to Florigene Europe in the United States. In this patent, the
explants or plant material transformed with Agrobacterium are limited
to stem, leaf, peduncle, petiole, meristem and shoot apex. The invention
discloses a method to regenerate a chrysanthemum shoot and a chrysanthemum plant
expressing an exogenous gene.
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5567599
- Earliest priority - 21 August 1990
- Filed - 26 January 1994
- Granted - 22 October 1996
- Expected expiry - 25 January 2014
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Title - Method for producing transformed chrysanthemum
plants
| Claim 1
A method for producing a chrysanthemum plant comprising an exogenous DNA
fragment, which chrysanthemum plant is capable of expressing a gene within the
exogenous DNA fragment, comprising the steps of: A) isolating an explant from
source material from a chrysanthemum plant, wherein said source material is
selected from the group consisting of leaf, stem, peduncle, petiole, meristem,
and shoot apex;
B) inoculating the explant from source material from the
chrysanthemum plant with a culture of Agrobacterium, which
Agrobacterium comprises an exogenous DNA fragment comprising a gene
under the control of a promoter capable of promoting the transcription of the
gene within the exogenous fragment, to obtain an inoculated explant;
C)
incubating the inoculated explant of step (B) on an incubation medium;
D)
culturing the incubated explant of step (C) on a regeneration medium to obtain
regenerated shoots of the chrysanthemum plant; and
E) culturing the
regenerated shoots of step (D) on a rooting medium to obtain a chrysanthemum
plant comprising the exogenous DNA fragment, in which the chrysanthemum plant is
capable of expressing the gene within the exogenous DNA fragment.
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Claim 60
A method for producing a chrysanthemum plant comprising an exogenous DNA
fragment using Agrobacterium as a vector, comprising the steps of:
A) incubating chrysanthemum plant source material on a pretreatment medium;
B) isolating a leaf explant from source material from the chrysanthemum
plant;
C) inoculating the explant from source material from the
chrysanthemum plant with a culture of Agrobacterium, which
Agrobacterium comprises an exogenous DNA fragment comprising a gene
under the control of a promoter capable of promoting the transcription of the
gene within the exogenous fragment, to obtain an inoculated explant;
D)
incubating the inoculated explant of step (C) on an incubation medium;
E)
culturing the incubated mixture of step (D) on a regeneration medium to obtain
regenerated shoots of the chrysanthemum plant; and
F) culturing the
regenerated shoots of step (E) on a rooting medium to obtain a chrysanthemum
plant comprising the exogenous DNA fragment.
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Production of a transformed chrysanthemum plant expressing a gene of interest
by transforming different explants with Agrobacterium carrying the
exogenous gene. The group of explants transformed include leaf, stem, peduncle,
petiole, meristem and shoot apex. To obtain a chrysanthemum plant, shoot
formation and rooting are induced.
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Florigene Europe B.V.
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Remarks
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National phase entry of
WO
1992/03041 in Australia (AU 84330/91) has
lapsed on 13 May 1993.
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Note: Patent information on this page was last updated on 14 March 2006.
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