Chrysanthemum - Patent granted to Florigene Europe B.V.

A method for producing a chrysanthemum plant comprising an exogenous DNA fragment, which chrysanthemum plant is capable of expressing a gene within the exogenous DNA fragment, comprising the steps of: A) isolating an explant from source material from a chrysanthemum plant, wherein said source material is selected from the group consisting of leaf, stem, peduncle, petiole, meristem, and shoot apex;
B) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
C) incubating the inoculated explant of step (B) on an incubation medium;
D) culturing the incubated explant of step (C) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant; and
E) culturing the regenerated shoots of step (D) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment, in which the chrysanthemum plant is capable of expressing the gene within the exogenous DNA fragment.

A method for producing a chrysanthemum plant comprising an exogenous DNA fragment using Agrobacterium as a vector, comprising the steps of:

A) incubating chrysanthemum plant source material on a pretreatment medium;
B) isolating a leaf explant from source material from the chrysanthemum plant;
C) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
D) incubating the inoculated explant of step (C) on an incubation medium;
E) culturing the incubated mixture of step (D) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant; and
F) culturing the regenerated shoots of step (E) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment.