The second strategy, known as positive selection, uses selective, non−toxic compounds to exploit the auxotrophies of the transformed material, i.e., the material is unable to regenerate and grow in the absence of an external supply of specific compounds. These methods of positive selection are based on complementing the transformed cells with a gene(s) that enables them[add a comment]
- to produce the essential substance themselves, or
- to be able to utilize a substrate, which confers a metabolic advantage over the non−transformed cells.
Examples of positive selection systems are a[add a comment]
- transgenic glucuronidase in combination with the use of cytokinin glucuronides or other phytohormone glucuronides, and
- transgenes such as phosphomannose isomerase and
xylose isomerase that confer the ability to metabolize
alternative carbohydrate sources such as mannose and xylose.
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