Main components of an antibiotic resistance system

The effectiveness of a particular antibiotic resistance system depends mainly on the following elements:[add a comment]

• The selective agent. It should fully inhibit growth of untransformed cells. The lowest concentration of the toxic compound that suppresses growth of the non−transformed cells and does not cause detrimental effects to the transformed ones is used.
• The resistance gene. Transcriptional and translational control signals fused to the resistance gene determine to a great extent the expression level of resistance. In addition, the gene sequence plays an important role as some are more compatible with animal or plant systems or subgroups of animals and plants, such as monocotyledonous or dicotyledonous plants.
• The material to be selected. In the case of plants, sensitivity to the selective agent depends on many factors, including the explant type, the developmental stage, tissue culture conditions and the genotype.

Apart from these factors, for an antibiotic resistance system to be efficient and useful the selectable marker gene should be expressible in a wide variety of cells and tissues, the background metabolic activity or resistance should be minimal or negligible, and a clear phenotypic change should be visible.[add a comment]

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