Antibiotic resistance genes in general - Patents granted to Monsanto

Antibiotic resistance genes in general
Patents granted to Monsanto

Actual granted independent claims

US 5034322
Claim 1 A chimeric gene capable of expressing a polypeptide in plant comprising in sequence: a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene; b) a structural DNA sequence encoding a polypeptide that permits the selection of transformed plant cells containing said chimeric gene by rendering said plant cells resistant to an amount of an antibiotic that would be toxic to non-transformed plant cells, said structural DNA sequence being heterologous with respect to the promoter region; and c) a 3' non-translated region of a gene naturally expressed in plants, said region encoding a signal sequence for polyadenylation of mRNA.
Claim 6 A chimeric gene comprising in sequence: a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene; b) a heterologous structural DNA sequence encoding a neomycin phosphotransferase; and c) a 3' non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.
Claim 31 A microorganism identified by ATCC Accession Number 39264.

Claim 1

A chimeric gene capable of expressing a polypeptide in plant comprising in sequence:

a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene;
b) a structural DNA sequence encoding a polypeptide that permits the selection of transformed plant cells containing said chimeric gene by rendering said plant cells resistant to an amount of an antibiotic that would be toxic to non-transformed plant cells, said structural DNA sequence being heterologous with respect to the promoter region; and
c) a 3' non-translated region of a gene naturally expressed in plants, said region encoding a signal sequence for polyadenylation of mRNA.

Claim 6

A chimeric gene comprising in sequence: a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene;
b) a heterologous structural DNA sequence encoding a neomycin phosphotransferase; and
c) a 3' non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

Claim 31

A microorganism identified by ATCC Accession Number 39264.

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