Patents granted to Monsanto

npt gene as part of a chimeric gene construct for plant transformation

Actual granted independent claims

US 5034322
Claim 6 A chimeric gene comprising in sequence: A) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene; B) a heterologous structural DNA sequence encoding a neomycin phosphotransferase; and a 3' non-translated C) region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

Claim 6

A chimeric gene comprising in sequence:

A) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene;
B) a heterologous structural DNA sequence encoding a neomycin phosphotransferase; and a 3' non-translated
C) region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

US 6174724
Claim 8 A chimeric gene capable of expressing a polypeptide in plant cells comprising in sequence: A) a promoter region from a gene which is naturally expressed in plant cells; B) a 5' non-translated region; C) a structural coding sequence encoding a neomycin phosphotransferase polypeptide; and D) a 3' non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

US 6174724

Claim 8

A chimeric gene capable of expressing a polypeptide in plant cells comprising in sequence:

A) a promoter region from a gene which is naturally expressed in plant cells;
B) a 5' non-translated region;
C) a structural coding sequence encoding a neomycin phosphotransferase polypeptide; and
D) a 3' non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

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