Patents granted to the National Research Council of Canada

nptII gene as part of a bifunctional marker gene

Patents granted to the National Research Council of Canada

Actual granted independent claims

US 5639663
Claim 1 A fused gene comprising a first structural gene which encodes beta-glucuronidase activity, fused in frame and linked by an intergenic nucleotide sequence to a second structural gene which encodes neomycin phosphotransferase-II activity, and in a suitable host is capable of expressing a single, stable polypeptide translation product simultaneously having the combined activities of the first and second structural genes.
Claim 4 A nucleotide which comprises beta-glucuronidase and neomycin phosphotransferase-II structural genes fused in frame and linked by an intergenic nucleotide sequence, wherein the nucleotide encodes and, in a suitable host is capable of expressing a single stable polypeptide translation product having both beta-glucuronidase and neomycin phosphotransferase-II activities.

Claim 1

A fused gene comprising a first structural gene which encodes beta-glucuronidase activity, fused in frame and linked by an intergenic nucleotide sequence to a second structural gene which encodes neomycin phosphotransferase-II activity, and in a suitable host is capable of expressing a single, stable polypeptide translation product simultaneously having the combined activities of the first and second structural genes.

Claim 4

A nucleotide which comprises beta-glucuronidase and neomycin phosphotransferase-II structural genes fused in frame and linked by an intergenic nucleotide sequence, wherein the nucleotide encodes and, in a suitable host is capable of expressing a single stable polypeptide translation product having both beta-glucuronidase and neomycin phosphotransferase-II activities.

EP 583258 B1
Claim 1 A nucleotide which comprises gus and nptII structural genes respectively encoding proteins having ß-glucuronidase and neomycin phosphotransferase activities, wherein the structural genes are linked by an intergenic nucleotide sequence, and wherein the nucleotide encodes and, in a suitable host, is capable of expressing, one polypeptide having both said activities.

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