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Patent Lens > Technology Landscapes > Antibiotic Resistance Genes and their Uses in Plant Genetic Transformation
Patent Family No. 3
Selectable marker for development of vectors and transformation systems in plants.
Patents granted to Eli Lilly Co.
Actual granted independent claims
This patent was assigned to Novartis

US 5668298

Claim 1

A chimeric gene functional in a plant cell, which chimeric gene comprises: a) at a position 5' to coding region (c), a plant-expressible promoter sequence;
b) at a position 3' to coding region (c), a terminator signal sequence; and
c) a coding region of an aphIV gene, which coding region:

  1. encodes a functional hygromycin phosphotransferase enzyme; and
  2. is positioned between such plant-expressible promoter sequence (a) and such terminator signal sequence (b) so as to be expressible,
    wherein expression of such coding region in a plant cell confers resistance to hygromycin B on such plant cell and
    wherein such resistance to hygromycin B is capable of providing a basis for selection of such plant cell.
Claim 15

Plasmid pCEL40 shown in Fig. 2 and derivable by ligating the Bgl II digest of plasmid pCEL30, to the about 1.3 kb BamH I-Bgl II fragment of plasmid pOW20, such plasmids pCEL30 and pOW20 being obtainable from NRRL B-15915 and NRRL B-15838, respectively.

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